antibody

Guinea Pig Anti-EAAT2 (GLT-1) (extracellular) Antibody

AGC-022-GP

polyclonal

guinea-pigs

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

AGC-022-GP-CF_0.2 ml

Guinea Pig Anti-EAAT2 (GLT-1) (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

P31596

IHC WB

Human Rat Mouse

Guinea Pig

BLP-GC022

Mouse, human - identical

PBS pH7.4

Guinea pig total IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

2nd extracellular loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)KQLGPGKKNDEVS, corresponding to amino acid residues 151-163 of rat EAAT2

L-Glutamate (Glu) is an abundant amino acid that functions as the major excitatory neurotransmitter in the central nervous system. However, excess of Glu in the extracellular synaptic milieu leads to neuronal cell death by a process known as excitotoxicity.The extracellular levels of Glu are regulated by a family of high affinity plasma membrane transporters called excitatory amino acid transporters (EAATs) which are responsible for the re-uptake of Glu into the cells1,2.The EAAT family includes five members (EAAT1-EAAT5) that are members of the solute carrier family 1 (SLC1) of Na+-dependent transporters that also includes the neutral amino acid transporters ASCT1 and ASCT2.The Glu transporters present an unusual topology of eight transmembrane domains with two re-entrant loops and intracellular N- and C- termini. The transporter is likely assembled as a trimer where each monomer is a functional unit capable of binding the Glu substrate.The transport of Glu into the cells by the EAAT transporters is coupled to the Na+ and K+ electrochemical gradient as a driving force. Hence, the uptake of Glu is dependent on the co-transport of three Na+ and one H+ ions, and the counter transport of one K+ ion. In addition, to the well documented Glu uptake, the EAAT transporters show a Glu-independent Cl- conductance. The physiological significance of the Cl- current through the EAATs is currently unknown1,2.EAAT2 (also known as Glutamate Transporter-1, GLT-1) as well as EAAT1, is expressed predominantly in glia cells, while EAAT3, EAAT4 and EAAT5 are mostly expressed in neurons.As mentioned earlier, EAAT transporters represent the only (significant) mechanism for removal of glutamate from the extracellular fluid and hence are essential for the long-term maintenance of low and non-toxic concentrations of glutamate and the preservation of normal excitatory synaptic transmission.In addition to Glu uptake, the glutamate transporters provide glutamate for the synthesis of γ-Aminobutyric acid (GABA), glutathione and protein, suggesting an interactive role between EAATs and cellular metabolism1,2.Dysregulation of EAAT activities has been implicated in several neurodegenerative disorders such as Alzheimer's disease, traumatic brain injury, epilepsy and schizophrenia, suggesting that EAATs can be a useful target for the treatment of these conditions1,2.

IHC WB

Alomone Labs

Excitatory amino acid transporter 2, Sodium-dependent glutamate/aspartate transporter 2, Glutamate/Aspartate Transporter II, Solute Carrier Family 1 Member 2, SLC1A2

A Guinea Pig Polyclonal Antibody to Excitatory Amino Acid Transporter 2 (EAAT2, GLT-1)

Guinea Pig Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022-GP, formerly AGP-113) is a highly specific antibody directed against an extracellular epitope of the rat EAAT2 protein. The antibody can be used in western blot analysis and immunohistochemistry applications. It has been designed to recognize EAAT2 (GLT-1) from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-EAAT2 (GLT-1) (extracellular) Antibody (#AGC-022) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

BLP-GC022

NA

Excitatory amino acid transporter 2, Sodium-dependent glutamate/aspartate transporter 2, Glutamate/Aspartate Transporter II, Solute Carrier Family 1 Member 2, SLC1A2

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

SLC1A2

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:100

1:800