Anti-EAAT1 (GLAST) (extracellular)-FITC Antibody | Alomone Labs AGC-021-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-EAAT1 (GLAST) (extracellular)-FITC Antibody

catalog :

AGC-021-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of EAAT1 in live intact mouse BV-2 microglia cells: Cell surface detection of EAAT1 in live intact mouse BV-2 microglia cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-FITC. Green line: Cells + Anti-EAAT1 (GLAST) (extracellular)-FITC Antibody (#AGC-021-F), (5 µg).

product information

CAT :

AGC-021-F

SKU :

AGC-021-F_50 mcl

Product Name :

Anti-EAAT1 (GLAST) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P24942

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC021

Homology :

Mouse - identical; human 12/13 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

2nd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KQFKTSYEKRSFK, corresponding to amino acids 188-200 of rat EAAT1

Product Page - Scientific background :

Glutamate transporters play important roles in the termination of excitatory neurotransmission and in providing cells with glutamate for metabolic purposes.SLC1A3 (EAAT1) belongs to The SLC1 protein family which contains a range of human and prokaryotic glutamate and aspartate transporters. EAAT1 transports the acidic amino acids glutamate and aspartate against a concentration gradient generating a net movement of substrate and charge across the membrane. Glutamate transport by EAAT1 is coupled to the co-transport of three Na+ ions and one H+ ion, and the counter transport of one K+ ion1.EAAT1 is mainly expressed in astrocytes but can also be found in oligodendrocytes. EAAT1, together with EAAT2, are responsible for the bulk of glutamate uptake in the brain. They have several important roles, including the termination of the synaptic effects of glutamate, prevention of potentially toxic accumulation of extracellular glutamate, supply of glutamate to the astrocyte for synthesis of glutamine, which is involved in ammonia detoxification and in the glutamine-glutamate cycle and signaling of the metabolic needs of nearby neurons and activation of glycolysis in astrocytes2.EAAT1 has been implicated in the pathogenesis of episodic ataxia, a rare neurological disorder characterized by neonatal muscle stiffness and an exaggerated startle reflex induced by noise or touch3.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, SLC1A3

Short Description :

A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 1 (EAAT1, GLAST) Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-EAAT1 (GLAST) (extracellular) Antibody (#AGC-021) is a highly specific antibody directed against an epitope of rat Excitatory amino acid transporter 1. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize EAAT1 from rat, human and mouse samples. Anti-EAAT1 (GLAST) (extracellular)-FITC Antibody (#AGC-021-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as live cell direct flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, SLC1A3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC1A3

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AGC-021), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage