Anti-NMDAR2D (GRIN2D) (extracellular) Antibody | Alomone Labs AGC-020 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NMDAR2D (GRIN2D) (extracellular) Antibody

catalog :

AGC-020

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Temi S, Rudyk C, Armstrong J, Landrigan J, Dedek C, Salmaso N, et al. Differential expression of GluN2 NMDA receptor subunits in the dorsal horn of male and female rats. Channels (Austin). 2021;15:179-192 pubmed publisher
Bhandage A, Jin Z, Hellgren C, Korol S, Nowak K, Williamsson L, et al. AMPA, NMDA and kainate glutamate receptor subunits are expressed in human peripheral blood mononuclear cells (PBMCs) where the expression of GluK4 is altered by pregnancy and GluN2D by depression in pregnant women. J Neuroimmunol. 2017;305:51-58 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-NMDAR2D (GRIN2D) (extracellular) Antibody (#AGC-020), (1:200).3,4. Anti-NMDAR2D (GRIN2D) (extracellular) Antibody, preincubated with NMDAR2D/GRIN2D (extracellular) Blocking Peptide (#BLP-GC020).

image 2 :

Expression of NMDA receptor 2D in rat reticular thalamic nucleus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NMDAR2D (GRIN2D) (extracellular) Antibody (#AGC-020), (1:200). A. Staining (green) appears in a discreet population of cells (thin arrows). B. The same section was stained for parvalbumin (red) and cell nuclei were visualized with DAPI (blue). Cells positive for NR2D but negative for parvalbumin (thick arrow, shown in A) and cells positive for both NR2D and parvalbumin (vertical arrow) are both present.

image 3 :

Expression of NMDA receptor 2D in rat reticular thalamic nucleus - Immunohistochemical staining of immersion-fixed free floating rat brain frozen sections usingAnti-NMDAR2D (GRIN2D) (extracellular) Antibody(#AGC-020) (1:200). A. Staining (green) appears in a discreet population of cells (thin arrows). B. The same section was stained for parvalbumin (red) and cell nuclei were visualized with DAPI (blue). Cells positive for NR2D but negative for parvalbumin (thick arrow shown in A) and cells positive for both NR2D and parvalbumin (vertical arrow) are both present.

product information

CAT :

AGC-020

SKU :

AGC-020-CF_0.2 ml

Product Name :

Anti-NMDAR2D (GRIN2D) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62645

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC020

Homology :

Mouse, human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CRTQNRTHRGESLHR, corresponding to amino acid residues 345-359 of rat NMDAR2D

Product Page - Scientific background :

The NMDA receptors (NMDARs) are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson's and Alzheimer's disease.The expression and function of NMDA receptors are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

NMDA receptor 2D, NR2D, GluN2D, Ionotropic glutamate receptor subunit ε4, N-methyl-D-aspartate receptor subunit 2D

Short Description :

A Rabbit Polyclonal Antibody to NMDA Receptor 2D (GRIN2D)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat NMDA receptor 2D. Anti-NMDAR2D (GRIN2D) (extracellular) Antibody (#AGC-020) can be used in western blot and immunocytochemistry applications. It has been designed to recognize NR2D from rat, mouse, and human samples.

Negative Control :

BLP-GC020

Positive Control :

Synonyms :

NMDA receptor 2D, NR2D, GluN2D, Ionotropic glutamate receptor subunit ε4, N-methyl-D-aspartate receptor subunit 2D

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIN2D

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage