Anti-mGluR7 (extracellular) Antibody | Alomone Labs AGC-017 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-mGluR7 (extracellular) Antibody

catalog :

AGC-017

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Tomilin V, Mamenko M, Zaika O, Ren G, Marrelli S, Birnbaumer L, et al. TRPC3 determines osmosensitive [Ca2+]i signaling in the collecting duct and contributes to urinary concentration. PLoS ONE. 2019;14:e0226381 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 4) and rat (lanes 2 and 5) brain membranes and human CCF-STTGI astrocytoma (lanes 3 and 6) cell line lysate (1:200): - 1-3. Anti-mGluR7 (extracellular) Antibody (#AGC-017), (1:200).4-6. Anti-mGluR7 (extracellular) Antibody, preincubated with mGluR7 (extracellular) Blocking Peptide (#BLP-GC017).

image 2 :

Expression of mGluR7 in rat neocortex - Immunohistochemical staining of rat neocortex frozen sections using Anti-mGluR7 (extracellular) Antibody (#AGC-017). A. mGluR7 staining (green) appears in several neocortical neurons. B. Merge image showing mGluR7 staining together with cell nuclei (blue).

image 3 :

Cell surface detection of mGluR7 in live intact human T cell leukemia (Jurkat) cell line: - ___Unstained cells + goat-anti-rabbit-PE.___Cells +Anti-mGluR7 (extracellular)Antibody (#AGC-017) (1:20) + goat-anti-rabbit-PE.

product information

CAT :

AGC-017

SKU :

AGC-017-CF_0.2 ml

Product Name :

Anti-mGluR7 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35400

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC017

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

TISGSKKEDTDRKC, corresponding to amino acid residues 377-390 of rat mGluR7

Product Page - Scientific background :

Metabotropic glutamate receptors (mGluRs) belong to the super family of G-protein coupled receptors (seven transmembrane proteins). mGluRs are further divided into subfamilies: group I mGluRs (mGluR1 and mGluR5) which couple to Gq, thereby activating phospholipase C (PLC). Group II which include mGluR2 and mGluR3 couple to Gi, therefore inhibit the formation of adenylate cyclase. mGluR4, 6, 7, 8 which belong to group III also inhibit adenylate cyclase formation by coupling to Gi1.The C-terminus of these receptors has important functions in modulating their activity. This region is important for G-protein coupling, post-translational modifications like phosphorylation as well as protein-protein interactions. The C-terminal region is also subject to alternative splicing2. Indeed, mGluR7 can be detected as five different splice variants3.Like all group III metabotropic glutamate receptors, mGluR7 is localized presynaptically. It is highly expressed in the central nervous system and could also be detected in some peripheral organs like hair cells and spiral ganglion cells of the inner ear3.As opposed to other group III metabotropic glutamate receptors, mGluR7 has very low affinity for its substrate L-glutamate, therefore, only under high synaptic activity is the receptor active. This enables the receptor to act as a negative feedback loop by inhibiting the release of L-glutamate3,4.mGluR7 may play a molecular role in the pathways leading to emotion and cognition4.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Metabotropic glutamate receptor 7, GRM7, mGlu7, Gprc1g

Short Description :

A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 7

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat mGluR7. Anti-mGluR7 (extracellular) Antibody (#AGC-017) can be used in western blot, immunohistochemistry, immunocytochemistry and indirect flow cytometry applications. It has been designed to recognize mGluR7 from human, rat and mouse samples.

Negative Control :

BLP-GC017

Positive Control :

Synonyms :

Metabotropic glutamate receptor 7, GRM7, mGlu7, Gprc1g

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRM7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage