Anti-mGluR4 (extracellular) Antibody | Alomone Labs AGC-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-mGluR4 (extracellular) Antibody

catalog :

AGC-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-mGluR4 (extracellular) Antibody (#AGC-014), (1:200).3,4. Anti-mGluR4 (extracellular) Antibody, preincubated with mGluR4 (extracellular) Blocking Peptide (#BLP-GC014).

image 2 :

Peptide KPKGHPHMNSIR(C) corresponding to amino acid residues 33-44 of rat mGluR4 (AccessionP31423). Extracellular N-terminus.

image 3 :

Expression of mGluR4 in rat PC12 cells - Cell surface detection ofmGluR4 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells usingAnti-mGluR4 (extracellular)Antibody (#AGC-014) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B. Nuclei are stained with Hoechst 33342 (blue).

product information

CAT :

AGC-014

SKU :

AGC-014-CF_0.2 ml

Product Name :

Anti-mGluR4 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P31423

Applications :

IC IF LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC014

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

KPKGHPHMNSIR(C), corresponding to amino acid residues 33-44 of rat mGluR4

Product Page - Scientific background :

Metabotropic glutamate receptors (mGluRs) belong to the super family of G-protein coupled receptors (seven transmembrane proteins). mGluRs are further divided into subfamilies: group I mGluRs (mGluR1 and mGluR5) which couple to Gq, thereby activating phospholipase C (PLC). Group II which include mGluR2 and mGluR3 couple to Gi, therefore inhibit the formation of adenylate cyclase. mGluR4, 6, 7, 8 which belong to group III also inhibit adenylate cyclase formation by coupling to Gi1.The C-terminus of these receptors has important functions in modulating their activity. This region is important for G-protein coupling, post-translational modifications like phosphorylation as well as protein-protein interactions. The C-terminal region is also subject to alternative splicing2.mGluR4 is predominantly expressed presynaptically in neurons and in the cerebellum. A splice variant of the protein is expressed in taste buds. This variant lacks a large portion of the N-terminus and is normally referred to taste mGluR43,4. It is responsible (along with taste mGluR1) for mediating the taste of monosodium glutamate (or unami)2,4.mGluR4 knock out mice display impaired cerebellar synaptic plasticity as well as some learning disabilities5. In addition, this receptor has emerged as a target for the treatment of Parkinson's disease6.

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

Metabotropic glutamate receptor 4, GRM4, mGlu4, GPRC1D

Short Description :

A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 4

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat mGluR4. Anti-mGluR4 (extracellular) Antibody (#AGC-014) can be used in western blot and live cell imaging applications. It has been designed to recognize mGluR4 from rat, mouse and human samples.

Negative Control :

BLP-GC014

Positive Control :

Synonyms :

Metabotropic glutamate receptor 4, GRM4, mGlu4, GPRC1D

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRM4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage