Anti-mGluR3 (extracellular) Antibody | Alomone Labs AGC-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-mGluR3 (extracellular) Antibody

catalog :

AGC-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Zinni M, Mairesse J, Pansiot J, Fazio F, Iacovelli L, Antenucci N, et al. mGlu3 receptor regulates microglial cell reactivity in neonatal rats. J Neuroinflammation. 2021;18:13 pubmed publisher
Logan C, Bechard A, Hamor P, Wu L, Schwendt M, Knackstedt L. Ceftriaxone and mGlu2/3 interactions in the nucleus accumbens core affect the reinstatement of cocaine-seeking in male and female rats. Psychopharmacology (Berl). 2020;237:2007-2018 pubmed publisher
Pacheco N, Heaven M, Holt L, Crossman D, Boggio K, Shaffer S, et al. RNA sequencing and proteomics approaches reveal novel deficits in the cortex of Mecp2-deficient mice, a model for Rett syndrome. Mol Autism. 2017;8:56 pubmed publisher
Olivero G, Bonfiglio T, Vergassola M, Usai C, Riozzi B, Battaglia G, et al. Immuno-pharmacological characterization of group II metabotropic glutamate receptors controlling glutamate exocytosis in mouse cortex and spinal cord. Br J Pharmacol. 2017;: pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-mGluR3 (extracellular) Antibody (#AGC-012), (1:600).3,4. Anti-mGluR3 (extracellular) Antibody, preincubated with mGluR3 (extracellular) Blocking Peptide (#BLP-GC012).

image 2 :

Expression of mGluR3 in rat brain stem - Immunohistochemical staining of frozen brain stem sections using Anti-mGluR3 (extracellular) Antibody (#AGC-012), (1:100) followed by goat anti-rabbit AlexaFluor-555 secondary antibody (red). Staining is present in neuronal cell bodies (arrows) in the brainstem nuclei. Hoechst 33342 is used as the counterstain (blue).

image 3 :

Expression of mGluR3 in rat PC12 cells - Cell surface detection ofmGluR3 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells withAnti-mGluR3 (extracellular)Antibody (#AGC-012) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.

product information

CAT :

AGC-012

SKU :

AGC-012-CF_0.2 ml

Product Name :

Anti-mGluR3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P31422

Applications :

IC IF IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC012

Homology :

Mouse - identical; human, monkey - 14/15 amino residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GDHNFMRREIKIEGD, corresponding to amino acids 24-38 of rat mGluR3

Product Page - Scientific background :

L-glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group II of the metabotropic glutamate receptors includes the mGluR2 and mGluR3 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus that contains the glutamate binding site, and an intracellular C-terminus.1,2 mGluR2 and mGluR3 are coupled to Gi/G0 and hence inhibit cAMP formation following receptor activation.1,2mGluR3 is expressed in neurons and glia in many brain regions including the cerebral cortex, hippocampus and brain stem. mGluR3 expression is predominantly presynaptic consistent with its role as an inhibitory autoreceptor, that is, activation of mGluR3 by glutamate inhibits additional glutamate release from the same neurons.1,2Several lines of evidence suggest that mGluR3 is important for long term depression, glial function and neuroprotection. Some studies, though not all, have shown a genetic association of mGluR3 gene polymorphisms with psychosis and with schizophrenia-related phenotypes. Based in these findings, mGluR3 has been suggested as a therapeutic target for both psychosis and schizophrenia.3

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Metabotropic glutamate receptor 3, GRM3, mGlu3, GPRC1C

Short Description :

A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 3

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat mGluR3. The epitope is specific for mGluR3 and will not cross-react with the closely related mGluR2. Anti-mGluR3 (extracellular) Antibody (#AGC-012) can be used in western blot, live cell imaging and immunohistochemistry applications. It has been designed to recognize mGluR3 from rat and mouse samples.

Negative Control :

BLP-GC012

Positive Control :

Synonyms :

Metabotropic glutamate receptor 3, GRM3, mGlu3, GPRC1C

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRM3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage