Anti-mGluR2 (extracellular) Antibody | Alomone Labs AGC-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-mGluR2 (extracellular) Antibody

catalog :

AGC-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Hohoff C, Zhang M, Ambrée O, Kravchenko M, Buschert J, Kerkenberg N, et al. Deficiency of the palmitoyl acyltransferase ZDHHC7 impacts brain and behavior of mice in a sex-specific manner. Brain Struct Funct. 2019;224:2213-2230 pubmed publisher
Jin L, Wang M, Galvin V, Lightbourne T, Conn P, Arnsten A, et al. mGluR2 versus mGluR3 Metabotropic Glutamate Receptors in Primate Dorsolateral Prefrontal Cortex: Postsynaptic mGluR3 Strengthen Working Memory Networks. Cereb Cortex. 2018;28:974-987 pubmed publisher

image

image 1 :

Western blot analysis of rat cerebellum (lanes 1 and 3) and cortex (lanes 2 and 4) membranes: - 1,2. Anti-mGluR2 (extracellular) Antibody (#AGC-011), (1:400).3,4. Anti-mGluR2 (extracellular) Antibody, preincubated with mGluR2 (extracellular) Blocking Peptide (#BLP-GC011).

image 2 :

Expression of mGluR2 in rat brain - Immunohistochemical staining of perfusion-fixed brain frozen sections using Anti-mGluR2 (extracellular) Antibody (#AGC-011). A. mGluR2 (green) is visualized in the corpus callosum (CC) and hippocampal stratum oriens (OR). B. Glial fibrillary acidic protein (GFAP) (red), a marker of astrocytes. C. Merge of the two images demonstrates expression of mGluR2 in astrocytes. DAPI is used as the nuclear counterstain (blue).

image 3 :

Peptide SLSRGADGSRHIC corresponding to amino acids 109-121 of rat mGluR2 (AccessionP31421). Extracellular N-terminus.

product information

CAT :

AGC-011

SKU :

AGC-011-CF_0.2 ml

Product Name :

Anti-mGluR2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P31421

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC011

Homology :

Mouse, human, dog, monkey - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

SLSRGADGSRHIC, corresponding to amino acids 109-121 of rat mGluR2

Product Page - Scientific background :

L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group II of the metabotropic glutamate receptors includes the mGluR2 and mGluR3 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus domain that contains the glutamate binding site, and an intracellular C-terminus.1,2 mGluR2 and mGluR3 are coupled to Gi/Go and hence inhibit cAMP formation following receptor activation.1,2mGluR2 is widely distributed throughout the brain with high expression in several limbic areas including the cortex, hippocampus and amygdala. mGluR2 is localized primarily presynaptically, although postsynaptic localization has also been described.In line with its presynaptic localization, mGluR2 is thought to function as an autoreceptor in a negative feedback mechanism that suppress further release of glutamate from the cell on which it is expressed.The involvement of mGluR2 in neuronal excitability and synaptic transmission suggests that modulation of this receptor is a promising strategy for the treatment of neurological and neuropsychiatric disorders such as anxiety, schizophrenia, and pain.3

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Metabotropic glutamate receptor 2, GRM2, mGlu2, GPRC1B

Short Description :

A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat mGluR2. The epitope is specific for mGluR2 and will not cross-react with the closely related mGluR3 channel. Anti-mGluR2 (extracellular) Antibody (#AGC-011) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize mGluR2 from human, rat and mouse samples.

Negative Control :

BLP-GC011

Positive Control :

Synonyms :

Metabotropic glutamate receptor 2, GRM2, mGlu2, GPRC1B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRM2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage