Anti-GluR3 (GluA3) (extracellular) Antibody | Alomone Labs AGC-010 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GluR3 (GluA3) (extracellular) Antibody

catalog :

AGC-010

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, western blot knockout validation

more info or order :

Alomone Labs product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; 1:100; loading ...; fig 1d	GarcÃa HernÃ¡ndez S, Abe M, Sakimura K, Rubio M. Impaired auditory processing and altered structure of the endbulb of Held synapse in mice lacking the GluA3 subunit of AMPA receptors. Hear Res. 2017;344:284-294 pubmed publisher
	Niu F, Zhang B, Feng J, Mao X, Xu X, Dong J, et al. Protein profiling identified mitochondrial dysfunction and synaptic abnormalities after dexamethasone intervention in rats with traumatic brain injury. Neural Regen Res. 2021;16:2438-2445 pubmed publisher
	Peter S, Urbanus B, Klaassen R, Wu B, Boele H, Azizi S, et al. AMPAR Auxiliary Protein SHISA6 Facilitates Purkinje Cell Synaptic Excitability and Procedural Memory Formation. Cell Rep. 2020;31:107515 pubmed publisher
	Jiang S, Lin Y, Hsieh C. Electroacupuncture at Hua Tuo Jia Ji Acupoints Reduced Neuropathic Pain and Increased GABAA Receptors in Rat Spinal Cord. Evid Based Complement Alternat Med. 2018;2018:8041820 pubmed publisher
	Parkinson G, Chamberlain S, Jaafari N, Turvey M, Mellor J, Hanley J. Cortactin regulates endo-lysosomal sorting of AMPARs via direct interaction with GluA2 subunit. Sci Rep. 2018;8:4155 pubmed publisher
	Koszegi Z, Fiuza M, Hanley J. Endocytosis and lysosomal degradation of GluA2/3 AMPARs in response to oxygen/glucose deprivation in hippocampal but not cortical neurons. Sci Rep. 2017;7:12318 pubmed publisher

image

image 1 :

Western blot analysis of rat cerebellum lysates: - 1. Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010), (1:400).2. Anti-GluR3 (GluA3) (extracellular) Antibody, preincubated with GluR3/GluA3 (extracellular) Blocking Peptide (#BLP-GC010).

image 2 :

Expression of GRIA3 (GluR3) in mouse cerebellum - Immunohistochemical staining of frozen perfusion-fixed free floating sections of mouse cerebellum using Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010). A. Distribution of GRIA3 (red). B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that GRIA3 is localized to Bergmann glia (vertical arrow) and to Purkinje cell soma (horizontal arrow). DAPI is used as the counterstain (blue).

product information

CAT :

AGC-010

SKU :

AGC-010-CF_0.2 ml

Product Name :

Anti-GluR3 (GluA3) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P19492

Applications :

IC IF IHC IP LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC010

Homology :

Mouse, human, dog - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EKPFHLNYHVDHLD, corresponding to amino acid residues 60-73 of rat GluR3

Product Page - Scientific background :

L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors).The ligand-gated ion channel family consists of 15 members that have been subdivided into three families based on their pharmacological profile: the a-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) preferring receptors, the N-methyl-D-aspartate (NMDA) preferring and the kainate preferring receptors.The AMPA receptor subfamily includes four members AMPA1 to AMPA4, also known as GluR1 to GluR4 respectively.The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3 channels, although homotetramers can also been found.AMPA receptors are permeable to cations Na+, K+ and Ca2+. The Ca2+ permeability is dependent on the presence of AMPA2: whenever this subunit is present, the channel will be impermeable to Ca2+.1Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane potential.Synaptic strength that is defined as the level of post-synaptic depolarization can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning. Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has focused in understanding the mechanisms that govern AMPA receptor trafficking.2The exact physiological role of the AMPA3 receptor is not clear but a role in the modulation of oscillatory networks affecting sleep and breathing has been suggested.3

Applications may also work in :

IC IF IHC IP LCI WB

Supplier :

Alomone Labs

Target :

AMPA receptor 3, Glutamate receptor 3, Ionotropic glutamate receptor 3, AMPA-selective glutamate receptor 3, GRIA3, GluR-C, GluR-K3

Short Description :

A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 3 (GluR3)

Long Description :

Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010) is a highly specific antibody directed against an epitope of the rat ionotropic glutamate receptor 3. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, live cell imaging, and immunoprecipitation applications. It has been designed to recognize GluR3 from rat, mouse, and human samples.

Negative Control :

BLP-GC010

Positive Control :

Synonyms :

AMPA receptor 3, Glutamate receptor 3, Ionotropic glutamate receptor 3, AMPA-selective glutamate receptor 3, GRIA3, GluR-C, GluR-K3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIA3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage