Anti-GRIK2 (GluK2) Antibody | Alomone Labs AGC-009 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GRIK2 (GluK2) Antibody

catalog :

AGC-009

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Wu Q, Bai Y, Li W, Congdon E, Liu W, Lin Y, et al. Increased neuronal activity in motor cortex reveals prominent calcium dyshomeostasis in tauopathy mice. Neurobiol Dis. 2021;147:105165 pubmed publisher
Li Q, Ma T, Qiu Y, Cui W, Chen T, Zhang W, et al. Connexin 36 Mediates Orofacial Pain Hypersensitivity Through GluK2 and TRPA1. Neurosci Bull. 2020;36:1484-1499 pubmed publisher
Plotegher N, Perocheau D, Ferrazza R, Massaro G, Bhosale G, Zambon F, et al. Impaired cellular bioenergetics caused by GBA1 depletion sensitizes neurons to calcium overload. Cell Death Differ. 2020;27:1588-1603 pubmed publisher
Mihaly A. The Reactive Plasticity of Hippocampal Ionotropic Glutamate Receptors in Animal Epilepsies. Int J Mol Sci. 2019;20: pubmed publisher
Akiba R, Matsuyama T, Tu H, Hashiguchi T, Sho J, Yamamoto S, et al. Quantitative and Qualitative Evaluation of Photoreceptor Synapses in Developing, Degenerating and Regenerating Retinas. Front Cell Neurosci. 2019;13:16 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-GRIK2 (GluK2) Antibody (#AGC-009), (1:400).3,4. Anti-GRIK2 (GluK2) Antibody, preincubated with GRIK2/GluK2 Blocking Peptide (#BLP-GC009).

image 2 :

Peptide CSAMVEELRMSLK corresponding to amino acid residues858-870 of rat kainate receptor GluK2 (AccessionP42260). Intracellular C-terminus.

product information

CAT :

AGC-009

SKU :

AGC-009-CF_0.2 ml

Product Name :

Anti-GRIK2 (GluK2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P42260

Applications :

IC IF WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC009

Homology :

Human, mouse, dog - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CSAMVEELRMSLK, corresponding to amino acid residues 858-870 of rat kainate receptor GluK2

Product Page - Scientific background :

L-Glutamate, the major excitatory neurotransmitter in the central nervous system (CNS), operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein-coupled receptors).The ligand-gated ion channel family consists of fifteen members that have been subdivided into three families based upon their pharmacological profile: the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-preferring receptors, the N-methyl-D-aspartate (NMDA)-preferring receptors, and the kainate-preferring receptors.The kainate receptor subfamily consists of five members that have been further subdivided into two classes based upon structural homology and functional characteristics. GluR5, GluR6, and GluR7 receptor subunits share a high degree of homology and are able to form functional channels when expressed in heterologous systems. The KA-1 and KA-2 receptors are unable to form functional channels on their own, but when coexpressed with GluR5-7 receptor subunits, they form channels with high affinity for kainate.1,2Like AMPA receptors, the functional unit of endogenous kainate receptors is believed to be a tetramer, which can be either homomeric or heteromeric. Kainate receptors GluR5 and GluR6 (but not GluR7, KA-1, or KA-2) can undergo RNA editing; as in the AMPA receptor GluR2, a glutamine (Q) residue in the channel pore is edited to encode arginine (R) in the mature protein. Substitution of Q with R modulates the properties of the channel, producing channels with reduced single channel conductance and lower permeability to Ca2+.1,2GluR6 is expressed in the CNS in basal ganglia, cerebellum, and hipoccampus, as well as in the spinal cord. The exact physiological role of GluR6 is unclear, but a role in controlling neuronal excitability, synaptic integration, and synaptic plasticity has been proposed.

Applications may also work in :

IC IF WB

Supplier :

Alomone Labs

Target :

Glutamate receptor ionotropic kainate 2, Glutamate receptor 6, GluR6, GLUK6

Short Description :

A Rabbit Polyclonal Antibody to Kainate Receptor GluK2

Long Description :

Alomone Labs is pleased to offer Anti-GRIK2 (GluK2) Antibody (#AGC-009), a highly specific antibody directed against the intracellular C-terminus domain of the rat kainate receptor GluK2. The antibody can be used in western blot and immunofluorescence applications and will recognize GluR6 from rat, human and mouse origins.

Negative Control :

BLP-GC009

Positive Control :

Synonyms :

Glutamate receptor ionotropic kainate 2, Glutamate receptor 6, GluR6, GLUK6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIK2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage