Anti-GRIK1 (GluK1) (extracellular) Antibody | Alomone Labs AGC-008 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GRIK1 (GluK1) (extracellular) Antibody

catalog :

AGC-008

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Helm M, Dankovich T, Mandad S, Rammner B, Jähne S, Salimi V, et al. A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines. Nat Neurosci. 2021;24:1151-1162 pubmed publisher
Kim J, Lee S, Park K. CXCR4 Regulates Temporal Differentiation via PRC1 Complex in Organogenesis of Epithelial Glands. Int J Mol Sci. 2021;22: pubmed publisher
Mitchell R, Campbell G, Mikolajczak M, McGill K, Mahad D, Fleetwood Walker S. A Targeted Mutation Disrupting Mitochondrial Complex IV Function in Primary Afferent Neurons Leads to Pain Hypersensitivity Through P2Y1 Receptor Activation. Mol Neurobiol. 2019;: pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-GRIK1 (GluK1) (extracellular) Antibody (#AGC-008), (1:200).2. Anti-GRIK1 (GluK1) (extracellular) Antibody, preincubated with GRIK1/GluK1 (extracellular) Blocking Peptide (#BLP-GC008).

image 2 :

Expression of GluK1 in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-GRIK1 (GluK1) (extracellular) Antibody (#AGC-008), (1:400), followed by donkey anti rabbit-Cy3 antibody. GluK1 staining (red) appears in the granule layer and in the baskets under Purkinje cells (arrows). Nuclei were labeled with DAPI and appear in blue.

image 3 :

Expression of GluK1 in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sectionsusingAnti-GRIK1 (GluK1) (extracellular) Antibody(#AGC-008) (1:400) followed by donkey anti rabbit-Cy3 antibody. GluK1 staining (red) appears in the granule layer and in the baskets under Purkinje cells (arrows). Nuclei were labeled with DAPI and appear in blue.

product information

CAT :

AGC-008

SKU :

AGC-008-CF_0.2 ml

Product Name :

Anti-GRIK1 (GluK1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P22756

Applications :

IC IF IHC LCI WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC008

Homology :

Human, dog, monkey - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KASGEVSKHLYKVWKK, corresponding to amino acid residues 402-417 of rat kainate receptor GluK1

Product Page - Scientific background :

L-Glutamate, the major excitatory neurotransmitter in the central nervous system (CNS), operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein-coupled receptors).The ligand-gated ion channel family consists of fifteen members that have been subdivided into three families based upon their pharmacological profile: the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-preferring receptors, the N-methyl-D-aspartate (NMDA)-preferring receptors, and the kainate-preferring receptors.The kainate receptor subfamily consists of five members that have been further subdivided into two classes based upon structural homology and functional characteristics. GluR5, GluR6, and GluR7receptor subunits share a high degree of homology and are able to form functional channels when expressed in heterologous systems. The KA-1 and KA-2 receptors are unable to form functional channels on their own, but when coexpressed with GluR5-7 receptor subunits, they form channels with high affinity for kainate.1,2Like AMPA receptors, the functional unit of endogenous kainate receptors is believed to be a tetramer, which can be either homomeric or heteromeric. Kainate receptors GluR5 and GluR6 (but not GluR7, KA-1, or KA-2) can undergo RNA editing; as in the AMPA receptor GluR2, a glutamine (Q) residue in the channel pore is edited to encode arginine (R) in the mature protein. Substitution of Q with R modulates the properties of the channel, producing channels with reduced single channel conductance and lower permeability to Ca2+.1,2GluR5 is highly expressed in dorsal root ganglion (DRG) neurons of the peripheral nervous system (PNS) as well as in several structures of the CNS including the amygdala, the hipoccampus, and Purkinje cells of the cerebellum.GluR5 has been implicated in the modulation of synaptic transmission and synaptic plasticity in both the CNS and PNS, but the exact physiological function of GluR5 is not well understood. Nevertheless, an involvement in persistent pain and some types of learning has been proposed.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Glutamate receptor ionotropic kainate 1, Glutamate receptor 5, GluR5

Short Description :

A Rabbit Polyclonal Antibody to Kainate Receptor GluK1

Long Description :

Alomone Labs is pleased to offer Anti-GRIK1 (GluK1) (extracellular) Antibody (#AGC-008), a highly specific antibody directed against the extracellular N-terminus domain of the rat kainate receptor GluK1. The antibody can be used in western blot and immunofluorescence applications.

Negative Control :

BLP-GC008

Positive Control :

Synonyms :

Glutamate receptor ionotropic kainate 1, Glutamate receptor 5, GluR5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIK1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage