Anti-mGluR5 (extracellular) Antibody | Alomone Labs AGC-007 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-mGluR5 (extracellular) Antibody

catalog :

AGC-007

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; fig 7 immunoprecipitation; mouse; fig 7 immunocytochemistry; mouse; fig s7 western blot; mouse; fig 6	Wang X, Bey A, Katz B, Badea A, Kim N, David L, et al. Altered mGluR5-Homer scaffolds and corticostriatal connectivity in a Shank3 complete knockout model of autism. Nat Commun. 2016;7:11459 pubmed publisher
	Lee B, Lee K, Panda S, Gonzales Rojas R, Chong A, Bugay V, et al. Nanoparticle delivery of CRISPR into the brain rescues a mouse model of fragile X syndrome from exaggerated repetitive behaviours. Nat Biomed Eng. 2018;2:497-507 pubmed publisher
	Edfawy M, Guedes J, Pereira M, Laranjo M, Carvalho M, Gao X, et al. Abnormal mGluR-mediated synaptic plasticity and autism-like behaviours in Gprasp2 mutant mice. Nat Commun. 2019;10:1431 pubmed publisher
	Xie J, Chen S, Pan H. Presynaptic mGluR5 receptor controls glutamatergic input through protein kinase C-NMDA receptors in paclitaxel-induced neuropathic pain. J Biol Chem. 2017;292:20644-20654 pubmed publisher
	Niu Y, Dai Z, Liu W, Zhang C, Yang Y, Guo Z, et al. Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory. elife. 2017;6: pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:500).2. Anti-mGluR5 (extracellular) Antibody, preincubated with mGluR5 (extracellular) Blocking Peptide (#BLP-GC007).

image 2 :

Expression of mGluR5 in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat cerebellum sections using Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:50). mGluR5 (red) was detected in cerebellar Purkinje cells (vertical arrows) and in the molecular layer (horizontal arrows). Staining with mouse anti-parvalbumin (green) revealed co-localization in Purkinje but not in the molecular layer. Little staining of mGluR5 was detected in the granule layer. DAPI counterstain is used to visualize nuclei of all cells (blue).

image 3 :

Cell surface detection of mGluR5 in live intact mouse BV-2 microglia cells: - ___ Cells.___ Cells + goat-anti-rabbit-FITC.___ Cells +Anti-mGluR5 (extracellular) Antibody(#AGC-007) (5 g) + goat-anti-rabbit-FITC.

product information

CAT :

AGC-007

SKU :

AGC-007-CF_0.2 ml

Product Name :

Anti-mGluR5 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P31424

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC007

Homology :

Mouse - identical; human - 13/14 amino residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5

Product Page - Scientific background :

L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptors family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus domain and an intracellular C-terminus one. The N-terminus domain of group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.mGluR1 and mGluR5 receptors signal through Gq/G11 that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. More downstream signaling pathways include activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins has also been reported.mGluR5 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the cortex, basal ganglia and hippocampus.The mGluR5 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson's and Huntington's disease.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E

Short Description :

A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 5

Long Description :

Anti-mGluR5 (extracellular) Antibody (#AGC-007) is a highly specific antibody directed against an extracelluar epitope of rat mGluR5. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect live cell flow cytometry applications. It has been designed to recognize mGluR5 from rat, mouse, and human samples.

Negative Control :

BLP-GC007

Positive Control :

Synonyms :

Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRM5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage