product summary
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company name :
Alomone Labs
product type :
antibody
product name :
Guinea pig Anti-mGluR5 (extracellular) Antibody
catalog :
AGC-007-GP
clonality :
polyclonal
host :
guinea-pigs
conjugate :
nonconjugated
clone name :
NA
reactivity :
mouse, rat
application :
western blot
more info or order :
citations: 1
image
image 1 :
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: - 1,2. Guinea pig Anti-mGluR5 (extracellular) Antibody (#AGC-007-GP), (1:400).3,4. Guinea pig Anti-mGluR5 (extracellular) Antibody, preincubated with mGluR5 (extracellular) Blocking Peptide (#BLP-GC007). Following a broad screen of secondary antibodies, the following were used for this application:#106-035-006 (Jackson ImmunoResearch) #A7289 (Sigma)
product information
CAT :
AGC-007-GP
SKU :
AGC-007-GP-CF_0.2 ml
Product Name :
Guinea pig Anti-mGluR5 (extracellular) Antibody
Group Type :
Antibodies
Product Type :
Antibodies
Clonality :
Polyclonal
Accession :
P31424
Applications :
WB
Reactivity :
Rat Mouse
Host :
Guinea Pig
Blocking Peptide :
BLP-GC007
Homology :
Mouse - identical; human - 13/14 amino residues identical
Formulation :
PBS pH7.4
isotype :
Guinea pig total IgG
Peptide confirmation :
Confirmed by amino acid analysis and mass spectrometry
Reconstitution :
0.2 ml double distilled water (DDW).
Antibody Concentration After Reconstitut ... :
1 mg/ml
Storage After Reconstitution :
The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preservative :
No Preservative
Immunogen Location :
Extracellular, N-terminus
Label :
Unconjugated
Storage Before Reconstitution :
The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
Shipping and storage :
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
immunogen source species :
Rat
Sequence :
EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5
Product Page - Scientific background :
Metabotropic glutamate G protein-coupled receptors are divided into three groups on the basis of their sequence similarity, pharmacology and signal transduction mechanisms. mGluR5 is a class I mGlu receptor and is widely expressed throughout the cortex, hippocampus, striatum, caudate nucleus and nucleus accumbens, areas involved in emotion, motivation and cognition. mGluR5 is primarily located post-synaptically and is coupled to the Gq/11 pathway.mGluR5 is comprised of an extracellular N-terminus, an intracellular C-terminus and seven transmembrane α helices (TM1-7). Native ligands can bind to the receptor's extracellular domain. This extracellular binding is consistent with the narrow allosteric pocket the receptor possesses due to the tight configuration of its helical bundle1. mGluR5 is physically coupled to NMDA receptors by various scaffolding proteins including PSD-95, Shank, and Homer, as well as via a direct interaction. In addition, mGluR5 is biochemically coupled to NMDA receptor function via PKC. As a result of these molecular and biochemical interactions, activation of mGluR5 results in enhanced functionality of the NMDA receptor.mGluR5 is also involved in dendrogenesis and spineogenesis. mGluR5−/− knockout mice exhibit elevated spine densities irrespective of spine type or location along their dendritic trees in comparison to mGluR5+/− animals2.Recent studies have shown that mGluR5 is a critical mediator and a potential therapeutic target for the treatment of numerous disorders of the central nervous system, including depression, anxiety, drug addiction, chronic pain, Fragile X syndrome, Parkinson's disease, and gastroesophageal reflux disease3.
Applications may also work in :
WB
Supplier :
Alomone Labs
Target :
Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E
Short Description :
A Guinea Pig Polyclonal Antibody to Metabotropic Glutamate Receptor 5
Long Description :
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat mGluR5. Guinea pig Anti-mGluR5 (extracellular) Antibody (#AGC-007-GP, formerly AGP-090), raised in guinea pigs, can be used in western blot analysis. It has been designed to recognize mGluR5 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-mGluR5 (extracellular) Antibody (#AGC-007) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.
Negative Control :
BLP-GC007
Positive Control :
NA
Synonyms :
Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E
Lead Time :
1-2 Business Days
Country of origin :
Israel/IL
Applications key :
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Specifictiy :
GRM5
Form :
Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Comment :
Contact Alomone Labs for technical support and product customization
Species reactivity key :
H- Human, M- Mouse, R- Rat
Is Toxin :
No
Purity :
Affinity purified on immobilized antigen.
UNSPSC :
41116161
Clone :
NA
Standard quality control of each lot :
Western blot analysis
Antigen preadsorption control :
1 µg peptide per 1 µg antibody
Application Dilutions: Immunohistochemis ... :
Contact Alomone for information
Application Dilutions: Western blot wb :
1:400
more info or order :
company information
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com972 2 531 8002
headquarters: Israel
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