Anti-mGluR1 (extracellular) Antibody | Alomone Labs AGC-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-mGluR1 (extracellular) Antibody

catalog :

AGC-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Sexton R, Hachem A, Assi A, Bukhsh M, Gorski D, Speyer C. Metabotropic glutamate receptor-1 regulates inflammation in triple negative breast cancer. Sci Rep. 2018;8:16008 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:200).2. Anti-mGluR1 (extracellular) Antibody, preincubated with mGluR1 (extracellular) Blocking Peptide (#BLP-GC006).

image 2 :

Peptide (C)HEGVLNIDDYKIQMNK corresponding to amino acids 501-516 of rat mGluR1 (AccessionP23385). Extracellular N-terminus.

image 3 :

Cell surface detection ofmGluR1 in live intact Jurkat cells: - ___Unstained cells + goat-anti-rabbit-FITC.___Cells +Anti-mGluR1(extracellular)Antibody (#AGC-006)(10 g) + goat-anti-rabbit-FITC.

product information

CAT :

AGC-006

SKU :

AGC-006-CF_0.2 ml

Product Name :

Anti-mGluR1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P23385

Applications :

IC IF IFC IP LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC006

Homology :

Mouse, human, dog - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HEGVLNIDDYKIQMNK, corresponding to amino acids 501-516 of rat mGluR1

Product Page - Scientific background :

L-Glutamate is the major excitatory neurotransmitter in the central nervous system. It operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus and an intracellular C-terminus. The N-terminus domain of Group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.mGluR1 and mGluR5 receptors signal through Gq/G11, that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. Additional downstream signaling pathways include the activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins have also been reported.mGluR1 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the hippocampus, cerebellum, olfactory bulb and thalamus.The mGluR1 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson's and Huntington's disease.

Applications may also work in :

IC IF IFC IP LCI WB

Supplier :

Alomone Labs

Target :

Metabotropic glutamate receptor 1, GRM1, mGlu1, GPRC1a, PPP1R85, SCAR13

Short Description :

A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 1

Long Description :

Anti-mGluR1 (extracellular) Antibody (#AGC-006) is a highly specific antibody directed against the extracellular N-terminus domain of rat mGluR1. The antibody can be used in western blot and immunocytochemistry applications, and recognizes mGluR1 in rat, mouse and human samples.

Negative Control :

BLP-GC006

Positive Control :

Synonyms :

Metabotropic glutamate receptor 1, GRM1, mGlu1, GPRC1a, PPP1R85, SCAR13

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRM1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage