Anti-GluR2 (GluA2) (extracellular) Antibody | Alomone Labs AGC-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GluR2 (GluA2) (extracellular) Antibody

catalog :

AGC-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - free floating section

more info or order :

Alomone Labs product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; mouse; 1:500; loading ...; fig 4a	Yeung J, Palpagama T, Tate W, Peppercorn K, Waldvogel H, Faull R, et al. The Acute Effects of Amyloid-Beta1-42 on Glutamatergic Receptor and Transporter Expression in the Mouse Hippocampus. Front Neurosci. 2019;13:1427 pubmed publisher
western blot; rat; 1:75; fig 2a	Egbenya D, Hussain S, Lai Y, Xia J, Anderson A, Davanger S. Changes in synaptic AMPA receptor concentration and composition in chronic temporal lobe epilepsy. Mol Cell Neurosci. 2018;92:93-103 pubmed publisher
	Ford L, Asok A, Tripp A, Parro C, Fitzpatrick M, de Solis C, et al. CPEB3 low-complexity motif regulates local protein synthesis via protein-protein interactions in neuronal ribonucleoprotein granules. Proc Natl Acad Sci U S A. 2023;120:e2114747120 pubmed publisher
	Getz A, Ducros M, Breillat C, Lampin Saint Amaux A, Daburon S, Fran xe7 ois U, et al. High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning. Sci Adv. 2022;8:eabm5298 pubmed publisher
	Metzdorf K, Fricke S, Balia M, Korte M, Zagrebelsky M. Nogo-A Modulates the Synaptic Excitation of Hippocampal Neurons in a Ca2+-Dependent Manner. Cells. 2021;10: pubmed publisher
	Li L, Chen S, Zhou M, Wang L, Li D, Chen H, et al. α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly. Cell Rep. 2021;36:109396 pubmed publisher
	Helm M, Dankovich T, Mandad S, Rammner B, Jähne S, Salimi V, et al. A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines. Nat Neurosci. 2021;24:1151-1162 pubmed publisher
	Elhussiny M, Carini G, Mingardi J, Tornese P, Sala N, Bono F, et al. Modulation by chronic stress and ketamine of ionotropic AMPA/NMDA and metabotropic glutamate receptors in the rat hippocampus. Prog Neuropsychopharmacol Biol Psychiatry. 2021;104:110033 pubmed publisher
	Salpietro V, Dixon C, Guo H, Bello O, Vandrovcova J, Efthymiou S, et al. AMPA receptor GluA2 subunit defects are a cause of neurodevelopmental disorders. Nat Commun. 2019;10:3094 pubmed publisher
	Haglerød C, Hussain S, Nakamura Y, Xia J, Haug F, Ottersen O, et al. Presynaptic PICK1 facilitates trafficking of AMPA-receptors between active zone and synaptic vesicle pool. Neuroscience. 2017;344:102-112 pubmed publisher
	Szczurowska E, Ergang P, KubovÃ¡ H, Druga R, Salaj M, MareÅ¡ P. Influence of early life status epilepticus on the developmental expression profile of the GluA2 subunit of AMPA receptors. Exp Neurol. 2016;283:97-109 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005), (1:200).2. Anti-GluR2 (GluA2) (extracellular) Antibody, preincubated with GluR2/GluA2 (extracellular) Blocking Peptide (#BLP-GC005).

image 2 :

Expression of AMPA Receptor 2 (GluA2) in rat cerebellum - Immnunohistochemical staining of rat cerebellum using Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005) . A. Co-localization of GluA2 (green) and calbindin D28-K (red) in Purkinje cell dendrites (vertical arrow). B. Distribution of calbindin D28-K. C. Distribution of GluA2. Note that some GluA2 positive fibers (horizontal arrow) that are probable Bergmann glial processes do not stain for GluA2. D. The molecular and Purkinje layer (blue).

image 3 :

Expression of GFAP in rat brain - Immunohistochemical staining of free-floating rat frozen brain sections using Anti-GFAP Antibody (#AFP-001) (1:2000). In rat parietal cortex GFAP immunoreactivity (green) appears in astrocytes (arrows). Nuclei are stained using DAPI as the counterstain (blue).

product information

CAT :

AGC-005

SKU :

AGC-005-CF_0.2 ml

Product Name :

Anti-GluR2 (GluA2) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P19491

Applications :

IC IF IHC IP LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC005

Homology :

Mouse - identical; human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

NVGNINNDKKDETYR(C), corresponding to amino acid residues 179-193 of rat GluR2

Product Page - Scientific background :

AMPA receptors are members of the glutamate receptor family of ion channels that also include the NMDA and Kainate receptors. The three subfamilies are named after the original synthetic agonists that were identified as selective ligands of each family.The α-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptor subfamily includes four members AMPA1-AMPA4 that are also known as GluR1-GluR4 respectively.The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being actually heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3, although homotetramers can also be found.AMPA receptors are permeable to cations Na+, K+ and Ca2+. The Ca2+ permeability is dependent on the presence of AMPA2. The Ca2+ permeability of AMPA2 subunit is determined by the presence of the amino acid arginine (R) at a critical site in the pore loop instead of glutamine (Q) present in the same site in the other AMPA subunits. A post-transcriptional process known as RNA editing determines the presence of this R. Since most AMPA2 subunits in the adult brain have undergone RNA editing and most AMPA receptors contain the AMPA2 subunit, most native AMPA receptors will be impermeable to Ca2+.Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane.Synaptic strength, defined as the level of post-synaptic depolarization, can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning.Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has been focused on understanding the mechanisms that govern AMPA receptor trafficking.

Applications may also work in :

IC IF IHC IP LCI WB

Supplier :

Alomone Labs

Target :

AMPA receptor 2, Glutamate receptor 2, Ionotropic glutamate receptor 2, AMPA-selective glutamate receptor 2, GRIA2, GluR-B, GluR-K2

Short Description :

A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 2 (GluR2)

Long Description :

Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005) is a highly specific antibody directed against an epitope of the rat ionotropic glutamate receptor 2. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunoprecipitation applications. It has been designed to recognize GluR2 from human, mouse, and rat samples.

Negative Control :

BLP-GC005

Positive Control :

Synonyms :

AMPA receptor 2, Glutamate receptor 2, Ionotropic glutamate receptor 2, AMPA-selective glutamate receptor 2, GRIA2, GluR-B, GluR-K2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIA2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage