Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody | Alomone Labs AGC-005-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody

catalog :

AGC-005-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Zanganeh P, Barton S, Lim K, Qian E, Crombie D, Bye C, et al. α-Amino-3-hydroxyl-5-methyl-4-isoxazole-propionate receptor and RNA processing gene dysregulation are early determinants of selective motor neuron vulnerability in a mouse model of amyotrophic lateral sclerosis. Brain Commun. 2022;4:fcac081 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005-GP), (1:500).2. Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody, preincubated with GluR2/GluA2 (extracellular) Blocking Peptide (#BLP-GC005).

product information

CAT :

AGC-005-GP

SKU :

AGC-005-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P19491

Applications :

IC IF LCI WB

Reactivity :

Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-GC005

Homology :

Mouse - identical; human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

NVGNINNDKKDETYR(C), corresponding to amino acid residues 179-193 of rat GluR2

Product Page - Scientific background :

AMPA receptors are members of the glutamate receptor family of ion channels that also include the NMDA and Kainate receptors. The three subfamilies are named after the original synthetic agonists that were identified as selective ligands of each family.The α-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptor subfamily includes four members AMPA1-AMPA4 that are also known as GluR1-GluR4 respectively.The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being actually heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3, although homotetramers can also be found.AMPA receptors are permeable to cations Na+, K+ and Ca2+. The Ca2+ permeability is dependent on the presence of AMPA2: whenever this subunit is present, the channel will be impermeable to Ca2+. The Ca2+ permeability of the AMPA2 subunit is determined by the presence of the amino acid arginine (R) at a critical site in the pore loop instead of glutamine (Q) present in the same site in the other AMPA subunits. A post-transcriptional process known as RNA editing determines the presence of this R. Since most AMPA2 subunits in the adult brain have undergone RNA editing and most AMPA receptors contain the AMPA2 subunit, most native AMPA receptors will be impermeable to Ca2+.Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane.Synaptic strength, defined as the level of post-synaptic depolarization, can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning.Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has been focused on understanding the mechanisms that govern AMPA receptor trafficking.

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

AMPA receptor 2, Glutamate receptor 2, Ionotropic glutamate receptor 2, AMPA-selective glutamate receptor 2, GRIA2, GluR-B, GluR-K2

Short Description :

A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 2 (GluR2)

Long Description :

Alomone Labs is pleased to offer an antibody directed against an extracellular epitope of the rat ionotropic glutamate receptor 2. Guinea pig Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005-GP, formerly AGP-073) raised in guinea pig can be used in western blot and live cell imaging applications. It has been designed to recognize GluR2 from human, mouse and rat samples. The antigen used to immunize guinea pigs is the same as Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-GC005

Positive Control :

Synonyms :

AMPA receptor 2, Glutamate receptor 2, Ionotropic glutamate receptor 2, AMPA-selective glutamate receptor 2, GRIA2, GluR-B, GluR-K2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIA2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500-1:600

more info or order :

Alomone Labs product webpage