Anti-GluR2 (GluA2) (extracellular)-ATTO Fluor-488 Antibody | Alomone Labs AGC-005-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GluR2 (GluA2) (extracellular)-ATTO Fluor-488 Antibody

catalog :

AGC-005-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Xu L, Gao F, Cheng S, Zhou Z, Li F, Miao Y, et al. Activated ephrinA3/EphA4 forward signaling induces retinal ganglion cell apoptosis in experimental glaucoma. Neuropharmacology. 2020;178:108228 pubmed publisher

image

image 1 :

Expression of AMPA Receptor 2 (GluR2) in mouse brain - Immunohistochemical staining of frozen free-floating of the molecular layer of the mouse cerebellum using Anti-GluR2 (GluA2) (extracellular)-ATTO Fluor-488 Antibody (#AGC-005-AG), (1:20). Both dendrites of Purkinje cells (arrows) were stained (green). DAPI counterstain (blue) helps define the layers: granule (G), Purkinje (P), and molecular (M).

image 2 :

Immuno-colocalization of GFAP and AQP4 in rat brain - Immunohistochemical staining of immersion-fixed free floating rat brain frozen sections using Anti-GFAP Antibody (#AFP-001) (1:2000) and Anti-Aquaporin 4 (AQP4) (300-314)-ATTO-594 Antibody(#AQP-014-AR) (1:100). A. In rat fornix GFAP immunoreactivity (green) appears in astrocytic processes. B. In same section AQP4 staining (red) is detected in blood vessels. C. Merge of panel A and panel B shows colocalization around a large blood vessel (arrows). Nuclei are stained using DAPI as the counterstain (blue).

product information

CAT :

AGC-005-AG

SKU :

AGC-005-AG_50 mcl

Product Name :

Anti-GluR2 (GluA2) (extracellular)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P19491

Applications :

IF IHC

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC005

Homology :

Mouse - identical; human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

NVGNINNDKKDETYR(C), corresponding to amino acid residues 179-193 of rat GluR2

Product Page - Scientific background :

AMPA receptors are members of the glutamate receptor family of ion channels that also include the NMDA and kainate receptors. The three subfamilies are named after the original synthetic agonists that were identified as selective ligands of each subfamily.The α-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptor subfamily includes four members, AMPA1-AMPA4, that are also known as GluR1-GluR4, respectively.The functional AMPA channel is believed to be a tetramer. Most neuronal AMPA receptors are heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3, although homotetramers are also found.AMPA receptors are permeable to Na+, K+, and Ca2+ cations. Permeability to Ca2+ is dependent upon the presence of AMPA2: whenever this subunit is present, the channel is impermeable to Ca2+. Ca2+ permeability of the AMPA2 receptor subunit is determined by the presence of arginine (R), as a result of RNA editing, at a critical site in the pore loop that is occupied by glutamine (Q) in the other AMPA subunits. Since most AMPA2 subunits in the adult brain have undergone RNA editing and most AMPA receptors contain the AMPA2 subunit, most native AMPA receptors are impermeable to Ca2+.Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that open only in the presence of both glutamate and a depolarized membrane.Synaptic strength, defined as the level of post-synaptic depolarization, can be long term (hence the term long term potentiation, LTP) and therefore induces changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning.Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has been focused on understanding the mechanisms that govern AMPA receptor trafficking.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

AMPA receptor 2, Glutamate receptor 2, Ionotropic glutamate receptor 2, AMPA-selective glutamate receptor 2, GRIA2, GluR-B, GluR-K2

Short Description :

A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 2 (GluR2) Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005) is a highly specific antibody directed against an epitope of the rat ionotropic glutamate receptor 2. The antibody can be used in western blot, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize GluR2 from human, mouse, and rat samples. Anti-GluR2 (GluA2) (extracellular)-ATTO Fluor-488 Antibody (#AGC-005-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-GluR2 (GluA2) (extracellular)-ATTO Fluor-488 Antibody has been tested in immunohistochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

AMPA receptor 2, Glutamate receptor 2, Ionotropic glutamate receptor 2, AMPA-selective glutamate receptor 2, GRIA2, GluR-B, GluR-K2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIA2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AGC-005), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:20

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage