Anti-GluR1 (GluA1) (extracellular) Antibody | Alomone Labs AGC-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GluR1 (GluA1) (extracellular) Antibody

catalog :

AGC-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:50; fig 2a	Egbenya D, Hussain S, Lai Y, Xia J, Anderson A, Davanger S. Changes in synaptic AMPA receptor concentration and composition in chronic temporal lobe epilepsy. Mol Cell Neurosci. 2018;92:93-103 pubmed publisher
	Wang L, Pang K, Zhou L, Cebri xe1 n Silla A, Gonz xe1 lez Granero S, Wang S, et al. A cross-species proteomic map reveals neoteny of human synapse development. Nature. 2023;622:112-119 pubmed publisher
	Kuhlmann N, Wagner Valladolid M, Quesada Ramírez L, Farrer M, Milnerwood A. Chronic and Acute Manipulation of Cortical Glutamate Transmission Induces Structural and Synaptic Changes in Co-cultured Striatal Neurons. Front Cell Neurosci. 2021;15:569031 pubmed publisher
	Movsisyan N, Pardo L. Kv10.1 Regulates Microtubule Dynamics during Mitosis. Cancers (Basel). 2020;12: pubmed publisher
	Korinek M, González González I, Smejkalova T, Hajdukovic D, Skrenkova K, Krusek J, et al. Cholesterol modulates presynaptic and postsynaptic properties of excitatory synaptic transmission. Sci Rep. 2020;10:12651 pubmed publisher
	Fukaya M, Sugawara T, Hara Y, Itakura M, Watanabe M, Sakagami H. BRAG2a Mediates mGluR-Dependent AMPA Receptor Internalization at Excitatory Postsynapses through the Interaction with PSD-95 and Endophilin 3. J Neurosci. 2020;40:4277-4296 pubmed publisher
	Li L, Yin N, Li X, Miao Y, Cheng S, Li F, et al. Rac1 Modulates Excitatory Synaptic Transmission in Mouse Retinal Ganglion Cells. Neurosci Bull. 2019;: pubmed publisher
	Oh J, Lim C, Yoo K, Park H, Park Y, Kim E, et al. Adenomatous polyposis coli-stimulated GEF 1 (Asef1) is a negative regulator of excitatory synaptic function. J Neurochem. 2018;147:595-608 pubmed publisher
	Ambroziak W, Fourie C, Montgomery J. SAP97-mediated rescue of NMDA receptor surface distribution in a neuronal model of Huntington's disease. Hippocampus. 2018;28:707-723 pubmed publisher
	Zhang L, JABLONSKI A, Mojsilovic Petrovic J, Ding H, Seeholzer S, Newton I, et al. SAP97 Binding Partner CRIPT Promotes Dendrite Growth In Vitro and In Vivo. Eneuro. 2017;4: pubmed publisher
	Renner M, Wang L, Levi S, Hennekinne L, Triller A. A Simple and Powerful Analysis of Lateral Subdiffusion Using Single Particle Tracking. Biophys J. 2017;113:2452-2463 pubmed publisher
	Oh J, Rhee S, Silva A, Lee Y, Kim H. Noonan syndrome-associated SHP2 mutation differentially modulates the expression of postsynaptic receptors according to developmental maturation. Neurosci Lett. 2017;649:41-47 pubmed publisher
	Lee Y, Ehninger D, Zhou M, Oh J, Kang M, Kwak C, et al. Mechanism and treatment for learning and memory deficits in mouse models of Noonan syndrome. Nat Neurosci. 2014;17:1736-43 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-GluR1 (GluA1) (extracellular) Antibody (#AGC-004), (1:200).3,4. Anti-GluR1 (GluA1) (extracellular) Antibody, preincubated with GluR1/GluA1 (extracellular) Blocking Peptide (#BLP-GC004).

image 2 :

Expression of GluR1 in mouse hippocampus - Immunohistochemical staining of mouse hippocampus with Anti-GluR1 (GluA1) (extracellular) Antibody (#AGC-004). GluR1 (green) is present in the stratum oriens (Or) and radiatum (Ra) but not in the pyramidal layer (P). Staining of the same section with mouse anti-parvalbumin (red) identifies the pyramidal layer.

image 3 :

Peptide RTSDSRDHTRVDWKR(C) corresponding to amino acid residues 271-285 of rat GluR1 (AccessionP19490). Extracellular N-terminus.

product information

CAT :

AGC-004

SKU :

AGC-004-CF_0.2 ml

Product Name :

Anti-GluR1 (GluA1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P19490

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC004

Homology :

Mouse - 14/15 amino acid residues identical; human - 12/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

RTSDSRDHTRVDWKR(C), corresponding to amino acid residues 271-285 of rat GluR1

Product Page - Scientific background :

AMPA receptors are members of the glutamate receptor family of ion channels that also include the NMDA and Kainate receptors. The three subfamilies are named after the original synthetic agonists that were identified as selective ligands of each family.The α-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptor subfamily includes four members AMPA1-AMPA4 that are also known as GluR1-GluR4 respectively.The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being actually heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3, although homotetramers can also be found.AMPA receptors are permeable to cations Na+, K+ and Ca2+. The Ca2+ permeability is dependent on the presence of AMPA2: whenever this subunit is present, the channel will be impermeable to Ca2+. The Ca2+ permeability of the AMPA2 subunit is determined by the presence of an arginine (R) at a critical site in the pore loop instead of a glutamine (Q) present in the same site in the other AMPA subunits. A post-transcriptional process known as RNA editing determines the presence of this R. Since most AMPA2 subunits in the adult brain have undergone RNA editing and most AMPA receptors contain the AMPA2 subunit, most native AMPA receptors will be impermeable to Ca2+.Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane.Synaptic strength, defined as the level of post-synaptic depolarization, can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning.Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has focused in understanding the mechanisms that govern AMPA receptor trafficking.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

AMPA Receptor 1, Glutamate receptor 1, Ionotropic glutamate receptor 1, AMPA-selective glutamate receptor 1, GRIA1, GluR-A, GluR-K1

Short Description :

A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 1 (GluR1)

Long Description :

Anti-GluR1 (GluA1) (extracellular) Antibody (#AGC-004) is a highly specific antibody directed against an extracellular epitope of the rat ionotropic glutamate receptor 1. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize GluR1 from human, mouse, and rat samples.

Negative Control :

BLP-GC004

Positive Control :

Synonyms :

AMPA Receptor 1, Glutamate receptor 1, Ionotropic glutamate receptor 1, AMPA-selective glutamate receptor 1, GRIA1, GluR-A, GluR-K1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage