Anti-NMDAR2B (GluN2B) (extracellular) Antibody | Alomone Labs AGC-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NMDAR2B (GluN2B) (extracellular) Antibody

catalog :

AGC-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 32

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:3000; fig 2d	Dong Y, Hsu F, Koziol White C, Stepanova V, Jude J, Gritsiuta A, et al. Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells. Sci Rep. 2021;11:8205 pubmed publisher
western blot; mouse; loading ...; fig 4I	Niu Y, Dai Z, Liu W, Zhang C, Yang Y, Guo Z, et al. Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory. elife. 2017;6: pubmed publisher
	Yang L, Wei M, Wang Y, Zhang J, Liu S, Liu M, et al. Rabphilin-3A undergoes phase separation to regulate GluN2A mobility and surface clustering. Nat Commun. 2023;14:379 pubmed publisher
	Gramuntell Y, Klimczak P, Coviello S, Pérez Rando M, Nacher J. Effects of Aging on the Structure and Expression of NMDA Receptors of Somatostatin Expressing Neurons in the Mouse Hippocampus. Front Aging Neurosci. 2021;13:782737 pubmed publisher
	Hedou E, Douceau S, Chevilley A, Varangot A, Thiebaut A, Triniac H, et al. Two-Chains Tissue Plasminogen Activator Unifies Met and NMDA Receptor Signalling to Control Neuronal Survival. Int J Mol Sci. 2021;22: pubmed publisher
	Hildrestrand G, Rolseth V, Kunath N, Suganthan R, Jensen V, Bugaj A, et al. NEIL1 and NEIL2 DNA glycosylases modulate anxiety and learning in a cooperative manner in mice. Commun Biol. 2021;4:1354 pubmed publisher
	Bach E, Morgan J, Ewin S, Barth S, Raab Graham K, Weiner J. Chronic Ethanol Exposures Leads to a Negative Affective State in Female Rats That Is Accompanied by a Paradoxical Decrease in Ventral Hippocampus Excitability. Front Neurosci. 2021;15:669075 pubmed publisher
	Tseng W, Reinhart V, Lanz T, Weber M, Pang J, Le K, et al. Schizophrenia-associated SLC39A8 polymorphism is a loss-of-function allele altering glutamate receptor and innate immune signaling. Transl Psychiatry. 2021;11:136 pubmed publisher
	Korinek M, González González I, Smejkalova T, Hajdukovic D, Skrenkova K, Krusek J, et al. Cholesterol modulates presynaptic and postsynaptic properties of excitatory synaptic transmission. Sci Rep. 2020;10:12651 pubmed publisher
	Park H, Choi Y, Jung H, Kim S, Lee S, Han H, et al. Splice-dependent trans-synaptic PTPδ-IL1RAPL1 interaction regulates synapse formation and non-REM sleep. EMBO J. 2020;39:e104150 pubmed publisher
	Gerhard D, Pothula S, Liu R, Wu M, Li X, Girgenti M, et al. GABA interneurons are the cellular trigger for ketamine's rapid antidepressant actions. J Clin Invest. 2019;: pubmed publisher
	Plotegher N, Perocheau D, Ferrazza R, Massaro G, Bhosale G, Zambon F, et al. Impaired cellular bioenergetics caused by GBA1 depletion sensitizes neurons to calcium overload. Cell Death Differ. 2020;27:1588-1603 pubmed publisher
	Sceniak M, Fedder K, Wang Q, Droubi S, Babcock K, Patwardhan S, et al. An autism-associated mutation in GluN2B prevents NMDA receptor trafficking and interferes with dendrite growth. J Cell Sci. 2019;132: pubmed publisher
	Carbonell A, Cho C, Tindi J, Counts P, Bates J, Erdjument Bromage H, et al. Haploinsufficiency in the ANKS1B gene encoding AIDA-1 leads to a neurodevelopmental syndrome. Nat Commun. 2019;10:3529 pubmed publisher
	Afonso P, De Luca P, Carvalho R, Cortes L, Pinheiro P, Oliveiros B, et al. BDNF increases synaptic NMDA receptor abundance by enhancing the local translation of Pyk2 in cultured hippocampal neurons. Sci Signal. 2019;12: pubmed publisher
	Choi Y, Park H, Jung H, Kweon H, Kim S, Lee S, et al. NGL-1/LRRC4C Deletion Moderately Suppresses Hippocampal Excitatory Synapse Development and Function in an Input-Independent Manner. Front Mol Neurosci. 2019;12:119 pubmed publisher
	Li L, Yin N, Li X, Miao Y, Cheng S, Li F, et al. Rac1 Modulates Excitatory Synaptic Transmission in Mouse Retinal Ganglion Cells. Neurosci Bull. 2019;: pubmed publisher
	Kang R, Wang L, Sanders S, Zuo K, Hayden M, Raymond L. Altered Regulation of Striatal Neuronal N-Methyl-D-Aspartate Receptor Trafficking by Palmitoylation in Huntington Disease Mouse Model. Front Synaptic Neurosci. 2019;11:3 pubmed publisher
	Ewin S, Morgan J, Niere F, McMullen N, Barth S, Almonte A, et al. Chronic Intermittent Ethanol Exposure Selectively Increases Synaptic Excitability in the Ventral Domain of the Rat Hippocampus. Neuroscience. 2019;398:144-157 pubmed publisher
	Fan Y, Guan S, Luo L, Li Y, Yang L, Zhou X, et al. Tetrahydroxystilbene glucoside relieves the chronic inflammatory pain by inhibiting neuronal apoptosis, microglia activation, and GluN2B overexpression in anterior cingulate cortex. Mol Pain. 2018;14:1744806918814367 pubmed publisher
	Morini R, Ferrara S, Perrucci F, Zambetti S, Pelucchi S, Marcello E, et al. Lack of the Actin Capping Protein, Eps8, Affects NMDA-Type Glutamate Receptor Function and Composition. Front Mol Neurosci. 2018;11:313 pubmed publisher
	Li Y, Kim R, Cho Y, Song W, Kim D, Kim K, et al. Lrfn2-Mutant Mice Display Suppressed Synaptic Plasticity and Inhibitory Synapse Development and Abnormal Social Communication and Startle Response. J Neurosci. 2018;38:5872-5887 pubmed publisher
	Zhang L, JABLONSKI A, Mojsilovic Petrovic J, Ding H, Seeholzer S, Newton I, et al. SAP97 Binding Partner CRIPT Promotes Dendrite Growth In Vitro and In Vivo. Eneuro. 2017;4: pubmed publisher
	Schweitzer B, Singh J, Fejtova A, Groc L, Heine M, Frischknecht R. Hyaluronic acid based extracellular matrix regulates surface expression of GluN2B containing NMDA receptors. Sci Rep. 2017;7:10991 pubmed publisher
	Ferreira J, Papouin T, Ladépêche L, Yao A, Langlais V, Bouchet D, et al. Co-agonists differentially tune GluN2B-NMDA receptor trafficking at hippocampal synapses. elife. 2017;6: pubmed publisher
	Altmüller F, Pothula S, Annamneedi A, Nakhaei Rad S, Montenegro Venegas C, Pina Fernández E, et al. Aberrant neuronal activity-induced signaling and gene expression in a mouse model of RASopathy. PLoS Genet. 2017;13:e1006684 pubmed publisher
	Joshi P, Gabrielli M, Ponzoni L, Pelucchi S, Stravalaci M, Beeg M, et al. Fingolimod Limits Acute Aβ Neurotoxicity and Promotes Synaptic Versus Extrasynaptic NMDA Receptor Functionality in Hippocampal Neurons. Sci Rep. 2017;7:41734 pubmed publisher
	Prieto G, Trieu B, Dang C, Bilousova T, Gylys K, Berchtold N, et al. Pharmacological Rescue of Long-Term Potentiation in Alzheimer Diseased Synapses. J Neurosci. 2017;37:1197-1212 pubmed publisher
	Antonelli R, De Filippo R, Middei S, Stancheva S, Pastore B, Ammassari Teule M, et al. Pin1 Modulates the Synaptic Content of NMDA Receptors via Prolyl-Isomerization of PSD-95. J Neurosci. 2016;36:5437-47 pubmed publisher
	Wang Y, Sánchez Mendoza E, Doeppner T, Hermann D. Post-acute delivery of memantine promotes post-ischemic neurological recovery, peri-infarct tissue remodeling, and contralesional brain plasticity. J Cereb Blood Flow Metab. 2017;37:980-993 pubmed publisher
	Konstantoudaki X, Chalkiadaki K, Tivodar S, Karagogeos D, Sidiropoulou K. Impaired synaptic plasticity in the prefrontal cortex of mice with developmentally decreased number of interneurons. Neuroscience. 2016;322:333-45 pubmed publisher
	Telezhkin V, Schnell C, Yarova P, Yung S, Cope E, Hughes A, et al. Forced cell cycle exit and modulation of GABAA, CREB, and GSK3Î² signaling promote functional maturation of induced pluripotent stem cell-derived neurons. Am J Physiol Cell Physiol. 2016;310:C520-41 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysates: - 1. Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003), (1:600).2. Anti-NMDAR2B (GluN2B) (extracellular) Antibody, preincubated with NMDAR2B/GluN2B (extracellular) Blocking Peptide (#BLP-GC003).

image 2 :

Expression of NMDA receptor 2B in rat hippocampus - Immunohistochemical staining of rat hippocampal CA1 frozen sections stained with Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003), (1:100). A. NMDAR2B (green) appears in the pyramidal layer of CA1. B. Staining of neurofilament 200 (red) identifies neuronal processes. C. Confocal merge demonstrates localization of NMDAR2B in cells and not in processes.

product information

CAT :

AGC-003

SKU :

AGC-003-CF_0.2 ml

Product Name :

Anti-NMDAR2B (GluN2B) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q00960

Applications :

IC IF IHC IP LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC003

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)NTHEKRIYQSNMLNR, corresponding to amino acid residues 323-337 of rat NMDA receptor 2B

Product Page - Scientific background :

The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson's and Alzheimer's disease.NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

Applications may also work in :

IC IF IHC IP LCI WB

Supplier :

Alomone Labs

Target :

NMDA receptor 2B, Ionotropic glutamate receptor subunit ε2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B

Short Description :

A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B)

Long Description :

Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, live cell imaging, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize GluN2B from human, rat, and mouse samples.

Negative Control :

BLP-GC003

Positive Control :

Synonyms :

NMDA receptor 2B, Ionotropic glutamate receptor subunit ε2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIN2B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage