Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody | Alomone Labs AGC-003-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody

catalog :

AGC-003-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Espana A, Seth H, J xe9 z xe9 quel J, Huang T, Bouchet D, Lepleux M, et al. Alteration of NMDA receptor trafficking as a cellular hallmark of psychosis. Transl Psychiatry. 2021;11:444 pubmed publisher
Johansson E, Bouchet D, Tamouza R, Ellul P, Morr A, Avignone E, et al. Human endogenous retroviral protein triggers deficit in glutamate synapse maturation and behaviors associated with psychosis. Sci Adv. 2020;6:eabc0708 pubmed publisher
Bland T, Zhu M, Dillon C, Sahin G, Rodriguez Llamas J, Appleyard S, et al. Leptin Controls Glutamatergic Synaptogenesis and NMDA-Receptor Trafficking via Fyn Kinase Regulation of NR2B. Endocrinology. 2020;161: pubmed publisher
Li L, Yin N, Li X, Miao Y, Cheng S, Li F, et al. Rac1 Modulates Excitatory Synaptic Transmission in Mouse Retinal Ganglion Cells. Neurosci Bull. 2019;: pubmed publisher

image

image 1 :

Multiplex staining of GluN2A and GluN2B in mouse deep cerebellar nucleus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (#AGC-003-AR), (1:60) and Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002), (1:200). A. Sections were incubated with Anti-NMDAR2A (GluN2A) (extracellular) Antibody, followed by goat anti-rabbit-Alexa-488 (green). B. The same sections were incubated with Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (red). C. Merge of A and B demonstrates the ubiquitous colocalization of the GluN2A and GluN2B subunits in cells with neuronal profiles in this nucleus. Arrows point at an example of NR2A and NR2B co-expression.

product information

CAT :

AGC-003-AR

SKU :

AGC-003-AR_50 mcl

Product Name :

Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q00960

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC003

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)NTHEKRIYQSNMLNR, corresponding to amino acid residues 323-337 of rat NMDA receptor 2B

Product Page - Scientific background :

The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson's and Alzheimer's diseases.NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

NMDA receptor 2B, Ionotropic glutamate receptor subunit ε2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B

Short Description :

A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B) Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, live cell imaging, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize GluN2B from human, rat, and mouse samples. Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (#AGC-003-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-GC003

Positive Control :

Synonyms :

NMDA receptor 2B, Ionotropic glutamate receptor subunit ε2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIN2B

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Electrophysiology

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AGC-003), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50-1:60

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage