Anti-NMDAR2A (GluN2A) (extracellular) Antibody | Alomone Labs AGC-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NMDAR2A (GluN2A) (extracellular) Antibody

catalog :

AGC-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - free floating section, other

more info or order :

Alomone Labs product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:1000; loading ...; fig 2c	Dong Y, Hsu F, Koziol White C, Stepanova V, Jude J, Gritsiuta A, et al. Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells. Sci Rep. 2021;11:8205 pubmed publisher
other; mouse; 1:200; loading ...; fig 1b	Al Abed A, Sellami A, Potier M, Ducourneau E, Gerbeaud Lassau P, Brayda Bruno L, et al. Age-related impairment of declarative memory: linking memorization of temporal associations to GluN2B redistribution in dorsal CA1. Aging Cell. 2020;19:e13243 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:500; loading ...; fig 8e	Yeung J, Palpagama T, Tate W, Peppercorn K, Waldvogel H, Faull R, et al. The Acute Effects of Amyloid-Beta1-42 on Glutamatergic Receptor and Transporter Expression in the Mouse Hippocampus. Front Neurosci. 2019;13:1427 pubmed publisher
immunocytochemistry; mouse; 1:100; loading ...; fig s2	Roshanravan H, Kim E, Dryer S. NMDA Receptors as Potential Therapeutic Targets in Diabetic Nephropathy: Increased Renal NMDA Receptor Subunit Expression in Akita Mice and Reduced Nephropathy Following Sustained Treatment With Memantine or MK-801. Diabetes. 2016;65:3139-50 pubmed publisher
	Yang L, Wei M, Wang Y, Zhang J, Liu S, Liu M, et al. Rabphilin-3A undergoes phase separation to regulate GluN2A mobility and surface clustering. Nat Commun. 2023;14:379 pubmed publisher
	Tseng W, Reinhart V, Lanz T, Weber M, Pang J, Le K, et al. Schizophrenia-associated SLC39A8 polymorphism is a loss-of-function allele altering glutamate receptor and innate immune signaling. Transl Psychiatry. 2021;11:136 pubmed publisher
	Johansson E, Bouchet D, Tamouza R, Ellul P, Morr A, Avignone E, et al. Human endogenous retroviral protein triggers deficit in glutamate synapse maturation and behaviors associated with psychosis. Sci Adv. 2020;6:eabc0708 pubmed publisher
	Bayraktar G, Yuanxiang P, Confettura A, Gomes G, Raza S, Stork O, et al. Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus. Neuropsychopharmacology. 2020;45:2120-2130 pubmed publisher
	Carbonell A, Cho C, Tindi J, Counts P, Bates J, Erdjument Bromage H, et al. Haploinsufficiency in the ANKS1B gene encoding AIDA-1 leads to a neurodevelopmental syndrome. Nat Commun. 2019;10:3529 pubmed publisher
	Li L, Yin N, Li X, Miao Y, Cheng S, Li F, et al. Rac1 Modulates Excitatory Synaptic Transmission in Mouse Retinal Ganglion Cells. Neurosci Bull. 2019;: pubmed publisher
	Tan C, Lu N, Wang C, Chen D, Sun N, Lyu H, et al. Endothelium-Derived Semaphorin 3G Regulates Hippocampal Synaptic Structure and Plasticity via Neuropilin-2/PlexinA4. Neuron. 2019;101:920-937.e13 pubmed publisher
	Rocchio F, Tapella L, Manfredi M, Chisari M, Ronco F, Ruffinatti F, et al. Gene expression, proteome and calcium signaling alterations in immortalized hippocampal astrocytes from an Alzheimer's disease mouse model. Cell Death Dis. 2019;10:24 pubmed publisher
	Fan Y, Guan S, Luo L, Li Y, Yang L, Zhou X, et al. Tetrahydroxystilbene glucoside relieves the chronic inflammatory pain by inhibiting neuronal apoptosis, microglia activation, and GluN2B overexpression in anterior cingulate cortex. Mol Pain. 2018;14:1744806918814367 pubmed publisher
	Li Y, Kim R, Cho Y, Song W, Kim D, Kim K, et al. Lrfn2-Mutant Mice Display Suppressed Synaptic Plasticity and Inhibitory Synapse Development and Abnormal Social Communication and Startle Response. J Neurosci. 2018;38:5872-5887 pubmed publisher
	Sibarov D, Bruneau N, Antonov S, Szepetowski P, Burnashev N, Giniatullin R. Functional Properties of Human NMDA Receptors Associated with Epilepsy-Related Mutations of GluN2A Subunit. Front Cell Neurosci. 2017;11:155 pubmed publisher
	Konstantoudaki X, Chalkiadaki K, Tivodar S, Karagogeos D, Sidiropoulou K. Impaired synaptic plasticity in the prefrontal cortex of mice with developmentally decreased number of interneurons. Neuroscience. 2016;322:333-45 pubmed publisher
	Telezhkin V, Schnell C, Yarova P, Yung S, Cope E, Hughes A, et al. Forced cell cycle exit and modulation of GABAA, CREB, and GSK3Î² signaling promote functional maturation of induced pluripotent stem cell-derived neurons. Am J Physiol Cell Physiol. 2016;310:C520-41 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysates: - 1. Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002), (1:600).2. Anti-NMDAR2A (GluN2A) (extracellular) Antibody, preincubated with NMDAR2A/GluN2A (extracellular) Blocking Peptide (#BLP-GC002).

image 2 :

Multiplex staining of GluN2A and GluN2B in mouse deep cerebellar nucleus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (#AGC-003-AR), (1:60) and Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002), (1:200). A. Sections were incubated with Anti-NMDAR2A (GluN2A) (extracellular) Antibody, followed by goat anti-rabbit-Alexa-488 (green). B. The same sections were incubated with Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (red). C. Merge of A and B demonstrates the ubiquitous colocalization of the GluN2A and GluN2B subunits in cells with neuronal profiles in this nucleus. Arrows point at an example of NR2A and NR2B co-expression.

product information

CAT :

AGC-002

SKU :

AGC-002-CF_0.2 ml

Product Name :

Anti-NMDAR2A (GluN2A) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q00959

Applications :

IC IF IFC IHC IP LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC002

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

GHSHDVTERELRN(C), corresponding to amino acid residues 41-53 of rat NR2A

Product Page - Scientific background :

The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson's and Alzheimer's disease.NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

Applications may also work in :

IC IF IFC IHC IP LCI WB

Supplier :

Alomone Labs

Target :

NMDA receptor 2A, NR2A, GRIN2A, Ionotropic glutamate receptor subunit ε1, N-methyl-D-aspartate receptor subunit 2A

Short Description :

A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A)

Long Description :

Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry. The antibody recognizes an extracellular epitope and is thus ideal for detecting GluN2A in living cells. It has been designed to recognize GluN2A from rat, mouse, and human samples.

Negative Control :

BLP-GC002

Positive Control :

Synonyms :

NMDA receptor 2A, NR2A, GRIN2A, Ionotropic glutamate receptor subunit ε1, N-methyl-D-aspartate receptor subunit 2A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIN2A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage