Anti-NMDAR1 (GluN1) (extracellular) Antibody | Alomone Labs AGC-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NMDAR1 (GluN1) (extracellular) Antibody

catalog :

AGC-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - free floating section

more info or order :

Alomone Labs product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; human; 1:400; loading ...; fig 3a	Coviello S, Gramuntell Y, Klimczak P, Varea E, Blasco Iba xf1 ez J, Crespo C, et al. Phenotype and Distribution of Immature Neurons in the Human Cerebral Cortex Layer II. Front Neuroanat. 2022;16:851432 pubmed publisher
immunohistochemistry; mouse; 1:300; loading ...; fig 1e	Kilonzo K, van der Veen B, Teutsch J, Schulz S, Kapanaiah S, Liss B, et al. Delayed-matching-to-position working memory in mice relies on NMDA-receptors in prefrontal pyramidal cells. Sci Rep. 2021;11:8788 pubmed publisher
immunocytochemistry; mouse; 1:100; loading ...; fig s2	Roshanravan H, Kim E, Dryer S. NMDA Receptors as Potential Therapeutic Targets in Diabetic Nephropathy: Increased Renal NMDA Receptor Subunit Expression in Akita Mice and Reduced Nephropathy Following Sustained Treatment With Memantine or MK-801. Diabetes. 2016;65:3139-50 pubmed publisher
	Tseng W, Reinhart V, Lanz T, Weber M, Pang J, Le K, et al. Schizophrenia-associated SLC39A8 polymorphism is a loss-of-function allele altering glutamate receptor and innate immune signaling. Transl Psychiatry. 2021;11:136 pubmed publisher
	Chen H, Vandorpe D, Xie X, Alper S, Zeidel M, Yu W. Disruption of Cav1.2-mediated signaling is a pathway for ketamine-induced pathology. Nat Commun. 2020;11:4328 pubmed publisher
	Ewin S, Morgan J, Niere F, McMullen N, Barth S, Almonte A, et al. Chronic Intermittent Ethanol Exposure Selectively Increases Synaptic Excitability in the Ventral Domain of the Rat Hippocampus. Neuroscience. 2019;398:144-157 pubmed publisher
	Makuch M, Wilson R, Al Diwani A, Varley J, Kienzler A, Taylor J, et al. N-methyl-D-aspartate receptor antibody production from germinal center reactions: Therapeutic implications. Ann Neurol. 2018;83:553-561 pubmed publisher
	Jezequel J, Johansson E, Dupuis J, Rogemond V, Gréa H, Kellermayer B, et al. Dynamic disorganization of synaptic NMDA receptors triggered by autoantibodies from psychotic patients. Nat Commun. 2017;8:1791 pubmed publisher
	Gobbo F, Marchetti L, Jacob A, Pinto B, Binini N, Pecoraro Bisogni F, et al. Activity-dependent expression of Channelrhodopsin at neuronal synapses. Nat Commun. 2017;8:1629 pubmed publisher
	Aloisi E, Le Corf K, Dupuis J, Zhang P, Ginger M, Labrousse V, et al. Altered surface mGluR5 dynamics provoke synaptic NMDAR dysfunction and cognitive defects in Fmr1 knockout mice. Nat Commun. 2017;8:1103 pubmed publisher
	Oh J, Rhee S, Silva A, Lee Y, Kim H. Noonan syndrome-associated SHP2 mutation differentially modulates the expression of postsynaptic receptors according to developmental maturation. Neurosci Lett. 2017;649:41-47 pubmed publisher
	Lesept F, Chevilley A, Jezequel J, LadÃ©pÃªche L, Macrez R, Aimable M, et al. Tissue-type plasminogen activator controls neuronal death by raising surface dynamics of extrasynaptic NMDA receptors. Cell Death Dis. 2016;7:e2466 pubmed publisher
	Telezhkin V, Schnell C, Yarova P, Yung S, Cope E, Hughes A, et al. Forced cell cycle exit and modulation of GABAA, CREB, and GSK3Î² signaling promote functional maturation of induced pluripotent stem cell-derived neurons. Am J Physiol Cell Physiol. 2016;310:C520-41 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001), (1:600).2. Anti-NMDAR1 (GluN1) (extracellular) Antibody, preincubated with NMDAR1/GluN1 (extracellular) Blocking Peptide (#BLP-GC001).

image 2 :

Multiplex staining of NMDAR1 and CALHM1 in mouse hippocampal CA1 region - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001), (1:200) and Anti-CALHM1-ATTO Fluor-594 Antibody (#ACC-101-AR), (1:60). A. Sections were stained with Anti-NMDAR1 (GluN1) (extracellular) Antibody, followed by goat-anti-rabbit-Cy2 (green). Staining reveals expression in neurons of the pyramidal layer (an arrow points at the layer). B. The same section was incubated with Anti-CALHM1-ATTO Fluor-594 Antibody (red). C. Merge of the two images demonstrates colocalization of NMDAR1 and CALHM1 in pyramidal neurons.

image 3 :

Immuno-colocalization of GluN1 and CALHM1 in mouse hippocampal CA1 region - Immunohistochemical staining of perfusion-fixed frozen mouse brain sectionsusingAnti-NMDAR1 (GluN1) (extracellular) Antibody(#AGC-001) (1:200) and Anti-CALHM1-ATTO-594 Antibody (#ACC-101-AR) (1:60). A. Sections were stained with Anti-NMDAR1 (GluN1) (extracellular)Antibody followed by goat-anti-rabbit-Cy2 (green). Staining reveals expression in neurons of the pyramidal layer (an arrow points at the layer). B. The same section was incubated with Anti-CALHM1-ATTO-594 Antibody (red). C. Merge of the two images demonstrates colocalization of GluN1 and CALHM1 in pyramidal neurons.

product information

CAT :

AGC-001

SKU :

AGC-001-CF_0.2 ml

Product Name :

Anti-NMDAR1 (GluN1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35439

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC001

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

ETEKPRGYQMSTRLK(C), corresponding to amino acid residues 385-399 of rat NMDA receptor 1

Product Page - Scientific background :

The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson's and Alzheimer's disease.NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

NMDA receptor 1, Ionotropic glutamate receptor subunit ζ 1, N-methyl-D-aspartate receptor subunit 1, GRIN1

Short Description :

A Rabbit Polyclonal Antibody to NMDA Receptor 1 (GluN1)

Long Description :

Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, live cell imaging, and immunohistochemistry applications. It has been designed to recognize NMDA receptor 1 from rat, mouse, and human samples.

Negative Control :

BLP-GC001

Positive Control :

Synonyms :

NMDA receptor 1, Ionotropic glutamate receptor subunit ζ 1, N-methyl-D-aspartate receptor subunit 1, GRIN1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

LCI IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage