Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody | Alomone Labs AGC-001-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody

catalog :

AGC-001-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Gramuntell Y, Klimczak P, Coviello S, Pérez Rando M, Nacher J. Effects of Aging on the Structure and Expression of NMDA Receptors of Somatostatin Expressing Neurons in the Mouse Hippocampus. Front Aging Neurosci. 2021;13:782737 pubmed publisher
Chipman P, Fung C, Pazo Fernandez A, Sawant A, Tedoldi A, Kawai A, et al. Astrocyte GluN2C NMDA receptors control basal synaptic strengths of hippocampal CA1 pyramidal neurons in the stratum radiatum. elife. 2021;10: pubmed publisher
Langer K, M xfc ller L xe4 ngle A, Wempe J, Laube B. Analysis of M4 Transmembrane Segments in NMDA Receptor Function: A Negative Allosteric Modulatory Site at the GluN1 M4 is Determining the Efficiency of Neurosteroid Modulation. Front Pharmacol. 2021;12:769046 pubmed publisher
Nestor J, Arinuma Y, Huerta T, Kowal C, Nasiri E, Kello N, et al. Lupus antibodies induce behavioral changes mediated by microglia and blocked by ACE inhibitors. J Exp Med. 2018;215:2554-2566 pubmed publisher
Mikasova L, Xiong H, Kerkhofs A, Bouchet D, Krugers H, Groc L. Stress hormone rapidly tunes synaptic NMDA receptor through membrane dynamics and mineralocorticoid signalling. Sci Rep. 2017;7:8053 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1.2. Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001-GP), (1:200).3.4. Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody, preincubated with NMDAR1/GluN1 (extracellular) Blocking Peptide (#BLP-GC001).

image 2 :

Multiplex staining of NMDAR1 and M4 muscarinic acetylcholine receptor in mouse parietal cortex - Immunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using rabbit Anti-CHRM4 Antibody (#AMR-004), (1:400) and Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001-GP), (1:1200). A. M4 muscarinic receptor staining (green). B. NMDAR1 staining (red) in same section. C. Merge of the two images reveals several cells expressing both M4 muscarinic receptor and NMDAR1 (arrows). Cell nuclei were stained with DAPI (blue).

product information

CAT :

AGC-001-GP

SKU :

AGC-001-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35439

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-GC001

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

ETEKPRGYQMSTRLK(C), corresponding to amino acid residues 385-399 of rat NMDA receptor 1

Product Page - Scientific background :

The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson's and Alzheimer's disease.NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

NMDA receptor 1, Ionotropic glutamate receptor subunit ζ 1, N-methyl-D-aspartate receptor subunit 1, GRIN1

Short Description :

A Guinea Pig Polyclonal Antibody to NMDA Receptor 1 (GluN1)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat NMDA Receptor 1. Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001-GP, formerly AGP-046) raised in guinea pig can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting NMDAR1 in living cells. It has been designed to recognize NMDAR1 from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-GC001

Positive Control :

Synonyms :

NMDA receptor 1, Ionotropic glutamate receptor subunit ζ 1, N-methyl-D-aspartate receptor subunit 1, GRIN1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GRIN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600-1:1200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage