Anti-GABA(B) R2 Antibody | Alomone Labs AGB-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(B) R2 Antibody

catalog :

AGB-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Yue J, Wang X, Guo Y, Zheng K, Liu H, Hu L, et al. Anxiolytic effect of CPEB1 knockdown on the amygdala of a mouse model of inflammatory pain. Brain Res Bull. 2018;137:156-165 pubmed publisher
Liu Z, Wang F, Fischer G, Hogan Q, Yu H. Peripheral nerve injury induces loss of nociceptive neuron-specific G?i-interacting protein in neuropathic pain rat. Mol Pain. 2016;12: pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane: - 1. Anti-GABA(B) R2 Antibody (#AGB-002), (1:200).2. Anti-GABA(B) R2 Antibody, preincubated with GABA(B) R2 Blocking Peptide (#BLP-GB002).

image 2 :

Expression of GABA(B) receptor 2 in mouse hippocampus - Immunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R2 Antibody (#AGB-002 ), (1:100). A. GABBR2 staining (green) is detected in layers including the stratum lacunosum moleculare (SLM) and stratum radiatum of the CA3 layer (star). B. Staining with mouse anti-GAP43 antibody (red) sets apart the SLM. C. Confocal merge suggests the presence of GABBR2 in layers, but not in pyramidal neurons.

image 3 :

Expression of GABA(B) receptor 2 in mouse hippocampus - Immunohistochemical staining of mouse hippocampus frozen sectionsusingAnti-GABA(B) R2Antibody (#AGB-002 ) (1:100). A. GABBR2 staining (green)is detected in layers including the stratum lacunosum moleculare (SLM) and stratum radiatum of the CA3 layer (star). B. Staining with mouse anti-GAP43 antibody (red)sets apart the SLM. C. Confocal merge suggests the presence of GABBR2 in layers but not in pyramidal neurons.

product information

CAT :

AGB-002

SKU :

AGB-002-CF_0.2 ml

Product Name :

Anti-GABA(B) R2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O75899

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GB002

Homology :

Rat, mouse, bovine - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CKDPIEDINSPEHIQRRLSL, corresponding to amino acid residues 875-894 of human GABBR2

Product Page - Scientific background :

GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA(A) R and GABA(C) R, and the metabotropic GABA(B) receptor, [GABA(B) R].3 The latter belongs to the G-protein coupled receptor (GPCR) superfamily and mediates slow synaptic inhibition in the brain and spinal cord.1,4The functional GABA(B) receptor is a heterodimer consisting of two subunits, the GABA(B) R1 and the GABA(B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6GABA(B) R2 has been shown to be essential for trafficking and for G-protein binding while the GABA(B) R1 subunit has been demonstrated to be important for agonist and antagonist binding. GABA(B) R2 is highly expressed in the brain, especially in the cerebral cortex, thalamus, and hippocampus, spinal cord, and, to a lesser extent, in the testis.7

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid type B receptor subunit 2, GABA B receptor 2, GABABR2, Gb2, GABBR2, GPR51, GPRC3B, HG20

Short Description :

A Rabbit Polyclonal Antibody to GABA(B) Receptor 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human GABA(B) receptor 2. Anti-GABA(B) R2 Antibody (#AGB-002), can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABBR2 from human, rat, and mouse samples.

Negative Control :

BLP-GB002

Positive Control :

Synonyms :

γ-Aminobutyric acid type B receptor subunit 2, GABA B receptor 2, GABABR2, Gb2, GABBR2, GPR51, GPRC3B, HG20

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABBR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage