Anti-GABA(B) R1 (extracellular) Antibody | Alomone Labs AGB-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(B) R1 (extracellular) Antibody

catalog :

AGB-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Varela López E, del Valle Mondragon L, Castrejón Tellez V, Perez Torres I, Arenas A, Rojas F, et al. Role of the Transient Receptor Potential Vanilloid Type 1 (TRPV1) in the Regulation of Nitric Oxide Release in Wistar Rat Aorta. Oxid Med Cell Longev. 2021;2021:8531975 pubmed publisher
Gao Y, Zhou J, Zhu Y, Wang L, Kosten T, Zhang X, et al. Neuroadaptations of presynaptic and postsynaptic GABAB receptor function in the paraventricular nucleus in response to chronic unpredictable stress. Br J Pharmacol. 2017;174:2929-2940 pubmed publisher
Liu Z, Wang F, Fischer G, Hogan Q, Yu H. Peripheral nerve injury induces loss of nociceptive neuron-specific G?i-interacting protein in neuropathic pain rat. Mol Pain. 2016;12: pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001), (1:200).2. Anti-GABA(B) R1 (extracellular) Antibody, preincubated with GABA(B) R1 (extracellular) Blocking Peptide (#BLP-GB001).

image 2 :

Expression of GABA(B) receptor 1 in mouse hippocampus - Immunohistochemical staining of mouse hippocampus frozen sections using Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001), (1:100). A. GABBR1 staining (green) is detected in neurons in the CA3 field and in the dentate granule layer (short arrows), as well as in dendrites of CA3 pyramidal neurons (long arrows). B. Staining with mouse anti-GAP43 antibody (red) sets apart the stratum lacunosum moleculare (SLM). C. Confocal merge suggests the presence of GABBR1 in pyramidal neurons.

image 3 :

Peptide CRGEREVVGPKVRK corresponding to amino acid residues 66-79 of rat GABBR1 (AccessionQ9Z0U4). Extracellular N-terminus.

product information

CAT :

AGB-001

SKU :

AGB-001-CF_0.2 ml

Product Name :

Anti-GABA(B) R1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Z0U4

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GB001

Homology :

Mouse, human, canine, bovine - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CRGEREVVGPKVRK, corresponding to amino acid residues 66-79 of rat GABBR1

Product Page - Scientific background :

GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA(A) R and GABA(C) R, and the metabotropic GABA (B) receptor, [GABA(B) R].3 The latter belongs to the G-protein coupled receptor superfamily and mediates slow synaptic inhibition in the brain and spinal cord.1,4The functional GABA (B) receptor is a heterodimer consisting of two subunits, the GABA(B) R1 and the GABA(B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6 The GABA(B) R1 subunit was demonstrated to be important for agonist and antagonist binding, while GABA(B) R2 was shown to be essential for trafficking and for G-protein binding.To date, eight alternatively spliced isoforms of GABA (B) R1 have been proposed. These are named 1a-1h, of which 1a and 1b are the most prominent. Only 1a, 1b, and 1c appear to act as functional subunits.6,7 They are widespread, being expressed in the testis, stomach, spinal cord, and brain. Isoform 1b is also expressed in the kidney and liver.7

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid type B receptor subunit 1, GABA B receptor 1, GABABR1, Gb1, GABBR1, GPRC3A

Short Description :

A Rabbit Polyclonal Antibody to GABA(B) Receptor 1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat GABA(B) receptor 1. Anti-GABA(B) R1 (extracellular) Antibody (#AGB-001) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABBR1 from human, rat, and mouse samples.

Negative Control :

BLP-GB001

Positive Control :

Synonyms :

γ-Aminobutyric acid type B receptor subunit 1, GABA B receptor 1, GABABR1, Gb1, GABBR1, GPRC3A

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABBR1

AB Specifictiy :

Does not recognize alternative splice 1b.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage