Anti-GABA(A) α5 Receptor Antibody | Alomone Labs AGA-025 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) α5 Receptor Antibody

catalog :

AGA-025

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Ruiz Reig N, Garc xed a S xe1 nchez D, Schakman O, Gailly P, Tissir F. Inhibitory synapse dysfunction and epileptic susceptibility associated with KIF2A deletion in cortical interneurons. Front Mol Neurosci. 2022;15:1110986 pubmed publisher
Kreis A, Desloovere J, Suelves N, Pierrot N, Yerna X, Issa F, et al. Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission. Sci Rep. 2021;11:17600 pubmed publisher
Bhandage A, Olivera G, Kanatani S, Thompson E, Lore K, Varas Godoy M, et al. A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites. elife. 2020;9: pubmed publisher

image

image 1 :

Western blot analysis of rat hippocampus (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: - 1,2. Anti-GABA(A) α5 Receptor Antibody (#AGA-025), (1:400).3,4. Anti-GABA(A) α5 Receptor Antibody, preincubated with GABA(A) α5 Receptor Blocking Peptide (#BLP-GA025).

image 2 :

Expression of GABRA5 in mouse brain - Immunohistochemical staining of mouse hippocampus sections using Anti-GABA(A) α5 Receptor Antibody (#AGA-025), (1;600). A. GABRA5 staining (green). B. Merged image of GABRA5 (green) with nuclei staining using DAPI as the counterstain (blue). GABRA5 is detected in the pyramidal layer (Pyr) (horizontal arrows) and in some interneurons (vertical arrow).

product information

CAT :

AGA-025

SKU :

AGA-025-CF_0.2 ml

Product Name :

Anti-GABA(A) α5 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P19969

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA025

Homology :

Mouse - identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KKERELILNKSTN, corresponding to amino acid residues 364-376 of rat GABRA5

Product Page - Scientific background :

GABA(A) receptors are the main inhibitory neurotransmitter receptors in the mammalian brain. They respond to a wide variety of drugs including benzodiazepines. The entire GABA receptor family contains 19 known genes.GABA(A) receptors are mostly pentameric proteins composed of different subunits and each subunit has several subtypes. Half of each subunit consists of a hydrophilic extracellular N-terminal domain containing a Cys loop, followed by four transmembrane sequences (M1-M4). M2 lines the ion channel and between M3 and M4 there is a large intracellular loop involved in receptor modulation by phosphorylation. Five subunits form a complex and surround a central ion channel with all five M2 segments contributing to the channel1.The gene encoding the GABA(A) receptor subunit α-5 (also known as GABRA5) is mapped to chromosome 15q11-q13 (together with GABRB3 and GABRG3) and is contained within approximately 800 kb of the distal part of the imprinted Prader-Willi and Angleman syndrome region. The receptor is mostly expressed in the hippocampus and prefrontal cortex2. GABAR5 is anchored to neuronal extra synaptic regions by the cytosolic protein radixin.GABRA5 is implicated in the development of various social disorders. PET studies have shown reduced binding of a GABRA5 selective ligand in the amygdala and nucleus acumbens of people with Autism spectrum disorders (ASD). In addition, post-mortem analysis of brain tissue obtained from individuals with ASD shows reduced amounts of GABRA5 encoding DNA. Deletion of the GABRA5 gene in mice causes autism-like behavior3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid receptor type A subunit α5, GABRA5

Short Description :

A Rabbit Polyclonal Antibody to GABA(A) α5 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat GABA(A) α5 receptor. Anti-GABA(A) α5 Receptor Antibody (#AGA-025) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABRA5 from human, rat, and mouse samples.

Negative Control :

BLP-GA025

Positive Control :

Synonyms :

γ-Aminobutyric acid receptor type A subunit α5, GABRA5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRA5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage