Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody | Alomone Labs AGA-020 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody

catalog :

AGA-020

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat eye (lanes 1 and 3) and ARPE-19 (lanes 2 and 4): - 1,2. Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody (#AGA-020), (1:200).3,4. Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody, preincubated with GABA(A) ρ3 Receptor/GABRR3 (extracellular) Blocking Peptide (#BLP-GA020).

image 2 :

Expression of GABA(A) ?3 receptor in human ARPE-19 cells - Cell surface detection ofGABA(A) ?3 receptor in intact living human retinal epithelium (ARPE-19) cells. Extracellular staining of cells usingAnti-GABA(A) ?3 Receptor (GABRR3) (extracellular) Antibody(#AGA-020) (1:50) followed by goat anti-rabbit-AlexaFluor-594secondary antibody. B. Merge of A and live view of the cells.

product information

CAT :

AGA-020

SKU :

AGA-020-CF_0.2 ml

Product Name :

Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P50573

Applications :

IC IF LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA020

Homology :

Mouse - identical; human - 9/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CLSSPKQTRIRETR, corresponding to amino acid residues 31-44 of rat GABRR3

Product Page - Scientific background :

γ-Aminobutyric acid (GABA) is the most abundant inhibitory neurotransmitter. It is involved in roughly 40% of inhibitory synapses1,2.GABA acts through two receptors, GABA(A) and GABA(B). To date, nineteen different GABA(A) subunits have been identified and divided in eight subunits: α (1-6), β (1-3), γ (1-3), δ, ε, ρ (1-3), θ and π. For some of the subunits, alternative splicing further increases the number of existing receptor types. They all have extracellular N- and C-termini and four transmembrane domains1.Three ρ subunits have been detected: GABA(A) ρ1, GABA(A) ρ2 and GABA(A) ρ3. Like all GABA(A) receptors the ρ subunits also assemble into a pentameric structure forming a Cl- channel. However, in contrast to all other GABA(A) subunits they mostly form homomeric entities. The GABA(A) ρ subunits display different pharmacological characteristics and were therefore once referred to as GABA(C) receptors. GABA(A) and GABA(B) respectively respond to bicuculline and baclofen, whereas ρ subunits are insensitive to either drug3-7. In addition, ρ subunits also display different electrophysiological properties, and are significantly more sensitive to GABA3,5,6,8.ρ subunits are highly expressed in the retina and it was believed that they are only expressed in that area. They are however, also expressed in central and peripheral nervous systems, as well as in the gastrointestinal and cardiovascular systems1.

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid receptor subunit ρ3, GABA(C) receptor

Short Description :

A Rabbit Polyclonal Antibody to GABA(A) ρ3 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GABA(A) rho-3 subunit. Anti-GABA(A) ρ3 Receptor (GABRR3) (extracellular) Antibody (#AGA-020) can be used in western blot and live cell imaging applications. It has been designed to recognize GABA(A) ρ3 from human, rat and mouse samples.

Negative Control :

BLP-GA020

Positive Control :

Synonyms :

γ-Aminobutyric acid receptor subunit ρ3, GABA(C) receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRR3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage