antibody

Anti-GABA(A) π Receptor (extracellular) Antibody

AGA-019

polyclonal

domestic rabbit

nonconjugated

NA

mouse, rat

western blot, immunohistochemistry

AGA-019-CF_0.2 ml

Anti-GABA(A) π Receptor (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

O09028

IHC WB

Rat Mouse

Rabbit

BLP-GA019

Mouse - identical; human - 13/14 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Extracellular, N-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

Peptide (C)DGNDVEFSWLRGND, corresponding to amino acid residues 184-197 of rat GABA(A) Pi Receptor

The GABA(A) receptor (GABAAR) is a pentameric transmembrane protein, member of the Cys-loop family of neurotransmitter-gated ion channels. Its ligand is γ-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. Binding of GABA induces the opening of an anion-selective ion channel that is an integral part of the receptor protein. The opening of this channel enables the entrance or exit of Cl- ions according to the resting potential of the cell surroundings. This causes an inhibitory effect on neurotransmission by decreasing the chance of a successful action potential transmission1. GABAARs occur in all organisms that have a nervous system, and they are mostly located in neuronal tissues. Due to their wide distribution within the nervous system and their basic function, they play a role in virtually all brain functions.These pentameric receptors occur with diverse subunit composition, at least 19 GABAAR subunits are identified in the human genome and the heteromeric nature of GABAAR allows for an enormous range of theoretically possible subunit combinations. Yet, experimental evidence suggests that only a few dozen combinations exist in vivo2.Members of the cys-loop family of ion channels share a similar structure that consists of an extracellular N- and C-terminal domains, four helical membrane-spanning segments (M1, M2, M3, and M4) and a 15-residue disulfide-linked loop in the extracellular domain3.The pivotal role of GABAARs in all brain functions makes them a desirable drug target and an impressive range of clinically used therapeutics are known to bind to distinct sites found on GABAARs to modulate receptor function4.

IHC WB

Alomone Labs

GABA(A) receptor subunit pi, Gabrp, Gamma-aminobutyric acid receptor subunit pi

A Rabbit Polyclonal Antibody to GABA(A) π Receptor

Anti-GABA(A) π Receptor/GABRP (extracellular) Antibody (#AGA-019) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize GABRP from human, rat, and mouse samples.

BLP-GA019

NA

GABA(A) receptor subunit pi, Gabrp, Gamma-aminobutyric acid receptor subunit pi

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

GABRP

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

ICC

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:200

1:200