Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody | Alomone Labs AGA-018 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody

catalog :

AGA-018

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and Human CCF-STGI brain astrocytoma cell lysate (lanes 3 and 6): - 1-3. Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018), (1:500).4-6. Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody, preincubated with GABA(A) θ Receptor/GABRQ (extracellular) Blocking Peptide (#BLP-GA018).

image 2 :

Expression of of GABA(A) θ receptor in rat hypothalamus - Immunohistochemical staining of rat hypothalamus using Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018). A. GABRQ staining (red) is detected in the mammillary nucleus which is part of the posterior hypothalamus (arrows demarcate nucleus border). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge images of A and B.

product information

CAT :

AGA-018

SKU :

AGA-018-CF_0.2 ml

Product Name :

Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JLF1

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA018

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

DYRMHEKLWVPDC, corresponding to amino acid residues 131-143 of mouse GABRQ

Product Page - Scientific background :

The A-type receptors for γ-aminobutyric acid (GABA(A)) mediate the majority of fast inhibitory transmission in the mammalian central nervous system. Many clinically important drugs including benzodiazepines, barbiturates and general anesthetics act as positive allosteric modulators of this receptor1. GABA(A) receptors are pentameric ligand-gated ion-channels, composed of subunits from many subunit classes: α1-α6, β1-β4, γ1-γ4, δ, ε , θ, π and ρ1-ρ32. The arrangement of subunits around the channel is probably γβαβα counter-clockwise when viewed from the extracellular space, ε and π subunits can replace the γ and δ subunit within the pentamer, whereas the θ subunit could replace a β subunit3.The localized expression of this subunit is consistent with the fact that all GABA(A) receptor subunit genes have distinct expression patterns in the brain3. The θ subunit has been reported to be strongly expressed in the striatum and has been localized to the locus ceruleus4.Impairment of the γ-aminobutyric acid (GABA) signaling system is believed to partially account for behavioral and cognitive deficits associated with schizophrenia and mood disorders5. Reduction of GABA(A) mediated signal transmission has also been associated with anxiety, panic, impaired learning and memory6.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid receptor subunit θ, GABA(A) receptor subunit theta

Short Description :

A Rabbit Polyclonal Antibody to GABA(A) θ Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse γ-aminobutyric acid receptor subunit θ. Anti-GABA(A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize GABRQ from rat, mouse and human samples.

Negative Control :

BLP-GA018

Positive Control :

Synonyms :

γ-Aminobutyric acid receptor subunit θ, GABA(A) receptor subunit theta

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRQ

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage