Anti-GABA(A) ε Receptor (GABRE) (extracellular)-ATTO Fluor-633 Antibody | Alomone Labs AGA-015-FR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) ε Receptor (GABRE) (extracellular)-ATTO Fluor-633 Antibody

catalog :

AGA-015-FR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Multiplex staining of Neurexin 1α and GABA(A) ε Receptor in mouse cerebellum - Immunohistochemical staining of rat cerebellum frozen section using Anti-Neurexin 1α (extracellular)-ATTO Fluor-488 Antibody (#ANR-031-AG) and Anti-GABA(A) ε Receptor (GABRE) (extracellular)-ATTO Fluor-633 Antibody (#AGA-015-FR). A. Neurexin 1α staining (green). B. GABA(A) ε receptor staining (purple). C. Merge of the two images suggests extensive co-localization in Purkinje cells (arrow).

image 2 :

Western blot analysis of human U-87 MG glioblastoma: - 1. Anti-MRGPRE Antibody(#AMR-062) (1:400).2. Anti-MRGPRE Antibody preincubated with the negative control antigen.

product information

CAT :

AGA-015-FR

SKU :

AGA-015-FR_50 mcl

Product Name :

Anti-GABA(A) ε Receptor (GABRE) (extracellular)-ATTO Fluor-633 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9ES14

Applications :

IC IF IHC LCI

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA015

Homology :

Mouse - identical; human - 10/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

ATTO-633. Maximum absorption 629 nm; maximum fluorescence 657 nm. The fluorescence is excited most efficiently in the 610 - 645 nm range. This label is analogous to the dyes Alexa 647, Alexa 633 and Cy5 and can be used for direct flow cytometry (FACS) using the He:Ne laser.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RLRYNDTFETLILH, corresponding to amino acid residues 131-144 of rat GABRE

Product Page - Scientific background :

GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system1.The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily2. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells3. Binding of GABA to the GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity2.GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ1. Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes4. For example, the α4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain5.

Applications may also work in :

IC IF IHC LCI

Supplier :

Alomone Labs

Target :

GABA(A) receptor subunit epsilon, Gamma-aminobutyric acid receptor subunit epsilon

Short Description :

A Rabbit Polyclonal Antibody to GABA(A) ε Receptor Conjugated to the Fluorescent Dye ATTO-633

Long Description :

Anti-GABA(A) ε Receptor (GABRE) (extracellular) Antibody (#AGA-015) is a highly specific antibody directed against an extracellular epitope of the rat GABA(A) receptor ε subunit. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell imaging applications. It has been designed to recognize GABA(A) ε receptor from human, rat and mouse samples. Anti-GABA(A) ε Receptor (GABRE) (extracellular)-ATTO Fluor-633 Antibody (#AGA-015-FR) is directly labeled with an ATTO-633 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm. The fluorescence is excited most efficiently in the range 610 to 645 nm. This label is analogous to the well-known dyes Alexa 647, Alexa 633 and Cy5. Anti-GABA(A) ε Receptor (GABRE) (extracellular)-ATTO Fluor-633 Antibody has been tested in live cell imaging and immunohistochemistry applications. It is specially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-FR

Positive Control :

Synonyms :

GABA(A) receptor subunit epsilon, Gamma-aminobutyric acid receptor subunit epsilon

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRE

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AGA-015), and immunocytochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage