Anti-GABA(A) β3 Receptor Antibody | Alomone Labs AGA-013 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) β3 Receptor Antibody

catalog :

AGA-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and LNCaP cell line lysate (lanes 3 and 6): - 1-3. Anti-GABA(A) β3 Receptor Antibody (#AGA-013), (1:1000)4-6. Anti-GABA(A) β3 Receptor Antibody, preincubated with GABA(A) β3 Receptor Blocking Peptide (#BLP-GA013).

image 2 :

Expression of GABA(A) β3 in mouse cerebellum. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GABA(A) β3 Receptor Antibody (#AGA-013), (1:200), followed by goat anti-rabbit-Alexa-488. A. GABA(A) β3 immunoreactivity (green) appears in the cerebellar granule layer (G). B. Pre-incubation of the antibody with GABA(A) β3 Receptor Blocking Peptide (#BLP-GA013), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGA-013

SKU :

AGA-013-CF_0.2 ml

Product Name :

Anti-GABA(A) β3 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P63079

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA013

Homology :

Mouse - identical; human - 10/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GRYMGDRSIPHKK, corresponding to amino acid residues 413-425 of rat GABRB3

Product Page - Scientific background :

GABA(A) receptors belong to the cys-loop pentameric ligand-gated ion channel family. These receptors are inhibitory neurotransmitter receptors in the brain and in the mammalian central nervous system. They are responsible for mediating GABA action, a neurotransmitter. They are composed of five homologous subunits that are arranged around a central chloride (Cl-)-selective pore. The human genome encodes 19 different GABA(A) receptor subunits: α1-6, β1-3, γ1-3, δ, ε, ρ1-3, π, and θ1,2,3.The beta-3 subunit plays a crucial role in coordinating the network rhythms in the thalamus and olfactory bulb. The protein is responsible to controlling hyperexcitability, preventing seizures. GABA(A) β3 receptor expression is detected in dendritic regions of the hippocampus and dentate gyrus. The β3 subunit is also associated with slow GABAergic current kinetics3,4.Mutation studies of GABRB3 gene are detected in patients with infantile spasms and Lennox-Gastaut syndrome. In addition, mice lacking the β3 subunit manifest fast decay kinetics on inhibitory postsynaptic currents in thalamic reticular and neocortical neurons3,5.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid receptor subunit β3, GABAA receptor beta-3, GABRB3, EIEE43, ECA5

Short Description :

A Rabbit Polyclonal Antibody to GABA(A) β3 Receptor

Long Description :

Anti-GABA(A) β3 Receptor Antibody (#AGA-013) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize GABRB3 from human, rat, and mouse samples.

Negative Control :

BLP-GA013

Positive Control :

Synonyms :

γ-Aminobutyric acid receptor subunit β3, GABAA receptor beta-3, GABRB3, EIEE43, ECA5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRB3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage