Anti-GABA(A) β2 Receptor Antibody | Alomone Labs AGA-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) β2 Receptor Antibody

catalog :

AGA-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1-2. Anti-GABA(A) β2 Receptor Antibody (#AGA-012), (1:200). 3-4. Anti-GABA(A) β2 Receptor Antibody, preincubated with GABA(A) β2 Receptor Blocking Peptide (BLP-GA012).

image 2 :

Expression of GABA(A) Receptor Beta 2 in mouse hypothalamus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections Anti-GABA(A) β2 Receptor Antibody (#AGA-012), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the periventricular hypothalamus region, showed GABA(A) Receptor Beta 2 immunoreactivity (green) in periventricular glial processes (arrows). B. Pre-incubation of the antibody with GABA(A) β2 Receptor Blocking Peptide (BLP-GA012), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = 3rd ventricle.

product information

CAT :

AGA-012

SKU :

AGA-012-CF_0.2 ml

Product Name :

Anti-GABA(A) β2 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P63138

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA012

Homology :

Mouse, Human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KAGLPRHSFGRNALE, corresponding to amino acid residues 405 - 419 of rat GABRB2

Product Page - Scientific background :

GABA receptors have two main subtypes: GABA(A) and GABA(B) receptors based on their amino acid sequence and structure. The GABA(A) receptors are ionotropic receptors while the GABA(B) receptors are metabotropic G-protein coupled receptors1.GABA(A) receptors are ion channels opened when GABA or its selective agonist muscimol binds to the receptor. In contrast, when the competitive antagonists bicuculline or gabazine binds to the GABA(A) receptors, the GABA-evoked current is decreased. GABA(A) receptors are blocked with picrotoxin, a non-competitive blocker, that blocks the GABA(A) receptor pore. Benzodiazepines and barbiturates are positive allosteric modulators of GABA(A) receptors and can enhance the currents of these channels2-4.To date, 19 GABA(A) receptor subunits have been identified: α 1-6, β 1-3, γ1-3, δ, ε, θ, p and ρ 1-34. GABA(A)receptors are heteropentameric complexes formed by combinations of different GABA(A) receptor subunits 4.The major isoform of the GABA(A) receptor in the brain is composed of two α1, two β2, and one γ2 subunits that are encoded by the GABRA1, GABRB2, and GABRG2 genes respectively, accounting for 43% of the GABA receptors in the mammalian brain 5. It is then not surprising that GABA(A) Receptor Beta 2, has been associated with several neuropsychiatric disorders such as schizophrenia, bipolar disorder, epilepsy, autism spectrum disorder, Alzheimer's disease, frontotemporal dementia and depression 5.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Gamma-Aminobutyric Acid Receptor Subunit Beta-2, GABA(A) receptor subunit beta-2, GABA(A) Receptor Beta 2, GABRB2

Short Description :

A Rabbit Polyclonal antibody to GABA(A) β2 Receptor

Long Description :

Anti-GABA(A) β2 Receptor Antibody (#AGA-012) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABA(A) Receptor Beta 2 from rat, mouse and human samples.

Negative Control :

BLP-GA012

Positive Control :

Synonyms :

Gamma-Aminobutyric Acid Receptor Subunit Beta-2, GABA(A) receptor subunit beta-2, GABA(A) Receptor Beta 2, GABRB2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRB2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage