Anti-GABA(A) β1 Receptor Antibody | Alomone Labs AGA-010 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) β1 Receptor Antibody

catalog :

AGA-010

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-GABA(A) β1 Receptor Antibody (#AGA-010), (1:500).3,4. Anti-GABA(A) β1 Receptor Antibody, preincubated with GABA(A) β1 Receptor Blocking Peptide (#BLP-GA010).

image 2 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)brainmembranes: - 12.Anti-GABA(A) ?1 Receptor Antibody (#AGA-010) (1:500).34. Anti-GABA(A) ?1 Receptor Antibody preincubated with the negative control antigen.

image 3 :

Peptide (C)EKQHEVYWKVSEDK corresponding to amino acid residues 80-93 of mouse SEMA4D (AccessionO09126). Extracellular N-terminus.

product information

CAT :

AGA-010

SKU :

AGA-010-CF_0.2 ml

Product Name :

Anti-GABA(A) β1 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P15431

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA010

Homology :

Human - identical; mouse - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DQSANEKNKLEMNK, corresponding to amino acid residues 345-358 of rat GABRB1

Product Page - Scientific background :

γ-Aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the vertebrate central nervous system (CNS) mediates fast synaptic transmission via ionotropic GABA(A) receptors. These receptors are pentameric assemblies of individual subunits arranged around a central, chloride-conducting pore. To date, 16 human GABA(A) receptor subunit genes have been characterized and grouped together according to their amino acid similarity and termed: α(1-6), β(1-3), δ(1-3), γ, ε, θ, and π1. Each subunit has a large extracellular N-terminal domain, four hydrophobic membrane-spanning domains that form the channel region and a small extracellular C-terminus2. These subunits can combine in many different ways to form functional receptors, but most synaptic receptors contain two α subunits (α 1-3 isoforms), two β subunits, and a γ subunit arranged in the order γ-β-α-β-α3.The β1 subunit gene is located in the β1- α4- α2- γ1 gene cluster on chromosome 4, and is most highly expressed in the adult rat hippocampus4. GABA(A) R genes are postulated to play a role in neuronal maturation, synaptogenesis, and predisposition to neurological disease. Increases in GABA levels and changes in GABA(A) R subunit gene expression, including decreased β1 mRNA levels, have been observed in animal models of epilepsy5. Recent genetic studies have implicated GABA(A) β1 Receptor (GABRB1) in bipolar disorder, schizoaffective disorder and major depression6. In addition, GABRB1 is associated with the risk of alcohol dependence7.

Applications may also work in :

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid receptor subunit β1, GABAA receptor beta-1, GABRB1, GARB1

Short Description :

A Rabbit Polyclonal Antibody to GABA(A) β1 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat GABA(A) β1 receptor. Anti-GABA(A) β1 Receptor Antibody (#AGA-010) can be used in western blot analysis. It has been designed to recognize GABRB1 from rat, mouse and human samples.

Negative Control :

BLP-GA010

Positive Control :

Synonyms :

γ-Aminobutyric acid receptor subunit β1, GABAA receptor beta-1, GABRB1, GARB1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRB1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage