Anti-GABA(A) α4 Receptor (extracellular) Antibody | Alomone Labs AGA-008 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) α4 Receptor (extracellular) Antibody

catalog :

AGA-008

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and human CCF-STGI Brain astrocytoma cell line lysate (lanes 3 and 6): - 1-3. Anti-GABA(A) α4 Receptor (extracellular) Antibody (#AGA-008), (1:200).4-6. Anti-GABA(A) α4 Receptor (extracellular) Antibody, preincubated with GABA(A) α4 Receptor (extracellular) Blocking Peptide (#BLP-GA008).

image 2 :

Expression of GABA(A) α4 Receptor in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-GABA(A) α4 Receptor (extracellular) Antibody (#AGA-008). A. GABRA4 staining (green) appears in the molecular layer (Mol) and around the soma of Purkinje cells (arrow). B. Parvalbumin (red), a marker of Purkinje and interneuronal cells, is stained in the same section. C. Merge of the images demonstrates expression of GABRA4 around the soma of Purkinje cells. DAPI is used as the counterstain (blue).

image 3 :

Expression of GABA(A) ?4 Receptor in rat PC12 cells - Cell surface detection ofGABA(A) ?4 Receptor in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells usingAnti-GABA(A) ?4 Receptor (extracellular)Antibody (#AGA-008) (1:50) (red). B. Merge of A with the live view of the cell.

product information

CAT :

AGA-008

SKU :

AGA-008-CF_0.2 ml

Product Name :

Anti-GABA(A) α4 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P28471

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA008

Homology :

Mouse, rat - 13/14 amino acid residues identical; human - 10/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

NSKDEKLSPENFTR(C), corresponding to amino acid residues 37-50 of rat GABRA4 with replacement of C44 with serine

Product Page - Scientific background :

GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system1.The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic GABAB receptors (GABABR).GABAARs belong to the ligand-gated ion channel superfamily2. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells3. Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity2.GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ1. Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes4. For example, the α4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain5.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid receptor subunit α4, GABRA4

Short Description :

A Rabbit Polyclonal Antibody to GABA(A) α4 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GABA(A) α4 subunit. Anti-GABA(A) α4 Receptor (extracellular) Antibody (#AGA-008) can be used in western blot, immunohistochemistry and live cell imaging applications. It was designed to recognize GABRA4 from human, rat and mouse samples.

Negative Control :

BLP-GA008

Positive Control :

Synonyms :

γ-Aminobutyric acid receptor subunit α4, GABRA4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRA4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage