Anti-GABA(A) γ2 Receptor (extracellular) Antibody | Alomone Labs AGA-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) γ2 Receptor (extracellular) Antibody

catalog :

AGA-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; loading ...; fig 3a	Fricke S, Metzdorf K, Ohm M, Haak S, Heine M, Korte M, et al. Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling. Cell Rep. 2019;29:671-684.e6 pubmed publisher
	Wang Y, Jiang S, Liu X, Niu L, Ge X, Zhang J, et al. Degradation of TRPML1 in Neurons Reduces Neuron Survival in Transient Global Cerebral Ischemia. Oxid Med Cell Longev. 2018;2018:4612727 pubmed publisher
	Letellier M, Szíber Z, Chamma I, Saphy C, Papasideri I, Tessier B, et al. A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation. Nat Commun. 2018;9:3979 pubmed publisher
	Bazzurro V, Gatta E, Cupello A, Lange S, Robello M. Antisecretory Factor Modulates GABAA Receptor Activity in Neurons. J Mol Neurosci. 2018;64:312-320 pubmed publisher
	Yue J, Wang X, Guo Y, Zheng K, Liu H, Hu L, et al. Anxiolytic effect of CPEB1 knockdown on the amygdala of a mouse model of inflammatory pain. Brain Res Bull. 2018;137:156-165 pubmed publisher
	Cantaut Belarif Y, Antri M, Pizzarelli R, Colasse S, Vaccari I, Soares S, et al. Microglia control the glycinergic but not the GABAergic synapses via prostaglandin E2 in the spinal cord. J Cell Biol. 2017;216:2979-2989 pubmed publisher
	de Luca E, Ravasenga T, Petrini E, Polenghi A, Nieus T, Guazzi S, et al. Inter-Synaptic Lateral Diffusion of GABAA Receptors Shapes Inhibitory Synaptic Currents. Neuron. 2017;95:63-69.e5 pubmed publisher
	Shen D, Hernandez C, Shen W, Hu N, Poduri A, Shiedley B, et al. De novo GABRG2 mutations associated with epileptic encephalopathies. Brain. 2017;140:49-67 pubmed publisher
	Wang J, Shen D, Xia G, Shen W, Macdonald R, Xu D, et al. Differential protein structural disturbances and suppression of assembly partners produced by nonsense GABRG2 epilepsy mutations: implications for disease phenotypic heterogeneity. Sci Rep. 2016;6:35294 pubmed publisher
	Warner T, Shen W, Huang X, Liu Z, Macdonald R, Kang J. Differential molecular and behavioural alterations in mouse models of GABRG2 haploinsufficiency versus dominant negative mutations associated with human epilepsy. Hum Mol Genet. 2016;25:3192-3207 pubmed publisher
	Du Z, Chazalon M, Bestaven E, Leste Lasserre T, Baufreton J, Cazalets J, et al. Early GABAergic transmission defects in the external globus pallidus and rest/activity rhythm alteration in a mouse model of Huntington's disease. Neuroscience. 2016;329:363-79 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-GABA(A) γ2 Receptor (extracellular) Antibody (#AGA-005), (1:200). 2. Anti-GABA(A) γ2 Receptor (extracellular) Antibody, preincubated with GABA(A) γ2 Receptor (extracellular) Blocking Peptide (#BLP-GA005).

product information

CAT :

AGA-005

SKU :

AGA-005-CF_0.2 ml

Product Name :

Anti-GABA(A) γ2 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P18508

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA005

Homology :

Human, mouse, bovine - identical; chick - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

QKSDDDYEDYASNKT(C), corresponding to amino acid residues 39-53 of rat GABA(A) γ2 receptor

Product Page - Scientific background :

The neurotransmitter GABA (γ-aminobutyric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.1 There are two major types of GABA receptors: the ionotropic GABAA (GABAA R) and the metabotropic GABAB receptors. GABAA R belongs to the ligand gated ion channel superfamily.1,2 It is a heteropentamer, with all of its five subunits contributing to the pore formation. To date, eight subunit isoforms were cloned: α, β, γ, δ, ε, π, θ, and ρ.1The native GABAA receptor, in most cases, consists of 2α, 2β and 1γ subunit. Three γ subunits genes have been identified in mammals. The binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance, resulting in inhibition of neural activity.2,3 Recently, a genetic linkage between familial epilepsy syndrome and mutations in the γ2 subunit of the GABAA receptor have been demonstrated.4,5

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

GABRG2, Gamma-aminobutyric acid receptor subunit gamma-2

Short Description :

A Rabbit Polyclonal Antibody to GABA(A) γ2 Receptor

Long Description :

Anti-GABA(A) γ2 Receptor (extracellular) Antibody (#AGA-005) is a highly specific antibody directed against an epitope of rat GABRG2. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. The antibody recognizes an extracellular epitope and is thus suited for live cell imaging. It has been designed to recognize GABA(A) γ2 from human, rat, and mouse samples.

Negative Control :

BLP-GA005

Positive Control :

Synonyms :

GABRG2, Gamma-aminobutyric acid receptor subunit gamma-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRG2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage