Anti-GABA(A) α6 Receptor (extracellular) Antibody | Alomone Labs AGA-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) α6 Receptor (extracellular) Antibody

catalog :

AGA-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Kita K, Albergaria C, Machado A, Carey M, Muller M, Delvendahl I. GluA4 facilitates cerebellar expansion coding and enables associative memory formation. elife. 2021;10: pubmed publisher
Chen X, Zhang J, Song Y, Yang P, Yang Y, Huang Z, et al. Deficiency of anti-inflammatory cytokine IL-4 leads to neural hyperexcitability and aggravates cerebral ischemia-reperfusion injury. Acta Pharm Sin B. 2020;10:1634-1645 pubmed publisher
Martenson J, Yamasaki T, Chaudhury N, Albrecht D, Tomita S. Assembly rules for GABAA receptor complexes in the brain. elife. 2017;6: pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-GABA(A) α6 Receptor (extracellular) Antibody, (1:200).2. Anti-GABA(A) α6 Receptor (extracellular) Antibody, preincubated with GABA(A) α6 Receptor (extracellular) Blocking Peptide (#BLP-GA004).

image 2 :

Expression of GABRA6 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004). A. GABRA6 (red) appears in the granule layer (asterisk). B. Parvalbumin (green) appears in Purkinje cells and in the molecular layer. C. Merge of GABRA6 and parvalbumin indicates that GABRA6 is restricted to granule cells. DAPI is used as the counterstain (blue).

image 3 :

Western blot analysis of rat brain membranes: - 1.Anti-GABA(A) ?6 Receptor (extracellular)Antibody (1:200).2. Anti-GABA(A) ?6 Receptor (extracellular) Antibody preincubated with the control peptide antigen.

product information

CAT :

AGA-004

SKU :

AGA-004-CF_0.2 ml

Product Name :

Anti-GABA(A) α6 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P30191

Applications :

IC IF IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA004

Homology :

Mouse - identical; human - 17/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

QLEDEGNFYSENVSRILDN(C), corresponding to amino acid residues 20-37 of rat GABRA6

Product Page - Scientific background :

GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake and metabolism occur in the nervous system.1The GABA transmitter interacts with two major types of receptors: ionotropic GABA(A) and metabotropic GABA(B) receptors. The GABA(A) receptor belongs to the ligand gated ion channel superfamily.2The GABA(A) R, is a heteropentamer, with all of its five subunits contributing to the pore formation.To date, eight subunit isoforms were cloned: α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunit isoforms were described to exist in mammals (α1-α6). The native GABA(A) receptor, in most cases, consists of 2α, 2β and 1γ subunit. The α subunit is the most common subunit and is expressed ubiquitously. It determines the affinities for allosteric ligands shown by GABA(A)R.A possible role for the GABA(A) receptors in alcohol action has been derived from animal studies, in vitro cell models and human research which suggested an important role for GABA(A) α6 along with β2, α1 and γ2, in alcohol related phenotypes.3In Canavan disease (inherited leukodystrophy), low level of expression of GABA(A) α6 was reported.4

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid receptor type A subunit α6, GABRA6

Short Description :

A Rabbit Polyclonal Antibody to GABA(A) α6 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat GABA(A) α6 receptor. Anti-GABA(A) α6 Receptor (extracellular) Antibody (#AGA-004) can be used in western blot, immunohistochemical and live cell imaging applications. It has been designed to recognize GABRA6 from rat, mouse and human samples.

Negative Control :

BLP-GA004

Positive Control :

Synonyms :

γ-Aminobutyric acid receptor type A subunit α6, GABRA6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRA6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage