Anti-GABA(A) α3 Receptor (extracellular) Antibody | Alomone Labs AGA-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) α3 Receptor (extracellular) Antibody

catalog :

AGA-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:200; loading ...	Pandya M, Palpagama T, Turner C, Waldvogel H, Faull R, Kwakowsky A. Sex- and age-related changes in GABA signaling components in the human cortex. Biol Sex Differ. 2019;10:5 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; fig 4	Gao Y, Heldt S. Enrichment of GABAA Receptor Î±-Subunits on the Axonal Initial Segment Shows Regional Differences. Front Cell Neurosci. 2016;10:39 pubmed publisher
	Ruiz Reig N, Garc xed a S xe1 nchez D, Schakman O, Gailly P, Tissir F. Inhibitory synapse dysfunction and epileptic susceptibility associated with KIF2A deletion in cortical interneurons. Front Mol Neurosci. 2022;15:1110986 pubmed publisher
	Kreis A, Desloovere J, Suelves N, Pierrot N, Yerna X, Issa F, et al. Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission. Sci Rep. 2021;11:17600 pubmed publisher
	Michalettos G, Walter H, Antunes A, Wieloch T, Talhada D, Ruscher K. Effect of Anti-inflammatory Treatment with AMD3100 and CX3CR1 Deficiency on GABAA Receptor Subunit and Expression of Glutamate Decarboxylase Isoforms After Stroke. Mol Neurobiol. 2021;58:5876-5889 pubmed publisher
	Bhandage A, Olivera G, Kanatani S, Thompson E, Lore K, Varas Godoy M, et al. A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites. elife. 2020;9: pubmed publisher
	Alkemade A, de Hollander G, Miletić S, Keuken M, Balesar R, de Boer O, et al. The functional microscopic neuroanatomy of the human subthalamic nucleus. Brain Struct Funct. 2019;224:3213-3227 pubmed publisher
	Palpagama T, Sagniez M, Kim S, Waldvogel H, Faull R, Kwakowsky A. GABAA Receptors Are Well Preserved in the Hippocampus of Aged Mice. Eneuro. 2019;6: pubmed publisher
	Bhandage A, Kanatani S, Barragan A. Toxoplasma-Induced Hypermigration of Primary Cortical Microglia Implicates GABAergic Signaling. Front Cell Infect Microbiol. 2019;9:73 pubmed publisher
	Angueyra J, Kindt K. Leveraging Zebrafish to Study Retinal Degenerations. Front Cell Dev Biol. 2018;6:110 pubmed publisher
	Doshina A, Gourgue F, Onizuka M, Opsomer R, Wang P, Ando K, et al. Cortical cells reveal APP as a new player in the regulation of GABAergic neurotransmission. Sci Rep. 2017;7:370 pubmed publisher
	Arellano R, SÃ¡nchez GÃ³mez M, Alberdi E, Canedo Antelo M, Chara J, Palomino A, et al. Axon-to-Glia Interaction Regulates GABAA Receptor Expression in Oligodendrocytes. Mol Pharmacol. 2016;89:63-74 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-GABA(A) α3 Receptor (extracellular) Antibody (#AGA-003), (1:200).2. Anti-GABA(A) α3 Receptor (extracellular) Antibody preincubated with GABA(A) α3 Receptor (extracellular) Blocking Peptide (#BLP-GA003).

image 2 :

Expression of GABRA3 in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-GABA(A) α3 Receptor (extracellular) Antibody (#AGA-003), (green), (1:100). GABRA3 is localized to the molecular layer (asterisk) and a portion of the Purkinje cell body (arrows), which is outlined by axonal staining using mouse anti-neurofilament 200 (red).

image 3 :

Expression of GABRA3 in rat insulinoma cell line - Cell surface detection ofGABRA3 in intact living rat RIN-m cells usingAnti-GABA(A) ?3 Receptor (extracellular)Antibody (#AGA-003) (1:50) followed by goat anti-rabbit-AlexaFluor-550 secondary antibody. Extracellular staining (red) merged with live view of the cells.

product information

CAT :

AGA-003

SKU :

AGA-003-CF_0.2 ml

Product Name :

Anti-GABA(A) α3 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P34903

Applications :

IC IF IHC IP LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA003

Homology :

Rat, mouse - identical; bovine - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

QGESRRQEPGDFVKQ(C), corresponding to amino acid residues 29-43 of human GABRA3

Product Page - Scientific background :

The neurotransmitter GABA (γ-aminobutiric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.1 Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.2There are two major types of GABA receptors: the ionotropic GABAA receptors (GABAAR) and the metabotropic GABAB receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily.1,2GABAARs are heteropentamers, in which all five subunits contribute to formation of the pore. Eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunits isoforms (α1-α6) have been shown to exist in mammals. In most cases, the native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α3-subunit is highly expressed during development (along with α2 and α5) and then declines in adulthood, where the α1-subunit becomes predominant. The failure to complete this switch could be a major predispositional factor in the development of temporal lobe epilepsy.3

Applications may also work in :

IC IF IHC IP LCI WB

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid receptor type A subunit α3, GABRA3

Short Description :

A Rabbit Polyclonal Antibody to GABA(A) α3 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human GABA(A) α3 receptor. Anti-GABA(A) α3 Receptor (extracellular) Antibody (#AGA-003) can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is highly suited to detect GABRA3 in living cells. It has been designed to recognize GABRA3 from rat, mouse, and human samples.

Negative Control :

BLP-GA003

Positive Control :

Synonyms :

γ-Aminobutyric acid receptor type A subunit α3, GABRA3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRA3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage