Anti-GABA(A) α2 Receptor Antibody | Alomone Labs AGA-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) α2 Receptor Antibody

catalog :

AGA-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:200; loading ...	Pandya M, Palpagama T, Turner C, Waldvogel H, Faull R, Kwakowsky A. Sex- and age-related changes in GABA signaling components in the human cortex. Biol Sex Differ. 2019;10:5 pubmed publisher
	Hong T, Falcone C, Dufour B, Amina S, Castro R, Regalado J, et al. GABAARα2 is Decreased in the Axon Initial Segment of Pyramidal Cells in Specific Areas of the Prefrontal Cortex in Autism. Neuroscience. 2020;437:76-86 pubmed publisher
	Palpagama T, Sagniez M, Kim S, Waldvogel H, Faull R, Kwakowsky A. GABAA Receptors Are Well Preserved in the Hippocampus of Aged Mice. Eneuro. 2019;6: pubmed publisher
	Qiao L, Yang Y, Liu J, Zhu J, Tan L, Shi Y, et al. Contribution of GABAergic modulation in DRGs to electroacupuncture analgesia in incisional neck pain rats. J Pain Res. 2019;12:405-416 pubmed publisher
	Januzi L, Poirier J, Maksoud M, Xiang Y, Veldhuizen R, Gill S, et al. Autocrine GABA signaling distinctively regulates phenotypic activation of mouse pulmonary macrophages. Cell Immunol. 2018;332:7-23 pubmed publisher
	Yue J, Wang X, Guo Y, Zheng K, Liu H, Hu L, et al. Anxiolytic effect of CPEB1 knockdown on the amygdala of a mouse model of inflammatory pain. Brain Res Bull. 2018;137:156-165 pubmed publisher
	Skilbeck K, Johnston G, Hinton T. Long-lasting effects of early-life intervention in mice on adulthood behaviour, GABAA receptor subunit expression and synaptic clustering. Pharmacol Res. 2018;128:179-189 pubmed publisher
	Ortinski P, O Donovan B, Dong X, Kantor B. Integrase-Deficient Lentiviral Vector as an All-in-One Platform for Highly Efficient CRISPR/Cas9-Mediated Gene Editing. Mol Ther Methods Clin Dev. 2017;5:153-164 pubmed publisher
	Kannampalli P, Babygirija R, Zhang J, Poe M, Li G, Cook J, et al. Neonatal bladder inflammation induces long-term visceral pain and altered responses of spinal neurons in adult rats. Neuroscience. 2017;346:349-364 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-GABA(A) α2 Receptor Antibody (#AGA-002), (1:200). 2. Anti-GABA(A) α2 Receptor Antibody, preincubated with GABA(A) α2 Receptor Blocking Peptide (#BLP-GA002).

image 2 :

Expression of GABRA2 in mouse cerebellum - Immunohistochemical staining of mouse formalin-fixed frozen cerebellum sections with Anti-GABA(A) α2 Receptor Antibody (#AGA-002). The receptor subunit is localized mainly on the soma of Purkinje cells. Staining with mouse anti-parvalbumin (red) suggests that GABRA2 subunit is restricted to a sub-region of the Purkinje cell.

image 3 :

Alomone Labs KN-62 inhibits human P2X7 receptors expressed in HEK-293 cells. - Dose-response curve of hP2X7 inhibition by KN-62 (#K-120). Cells were loaded with Fluo-8 NW dye incubated with increasing concentrations of KN-62 and stimulated with 80 M BzATP. Changes in intracellular Ca2+following agonist application were detected as changes in maximum relative fluorescence (RLU) using FLIPRTETRA?. IC50was calculated at 2.6 M.

product information

CAT :

AGA-002

SKU :

AGA-002-CF_0.2 ml

Product Name :

Anti-GABA(A) α2 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P23576

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA002

Homology :

Mouse, human, bovine - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)TPEPNKKPENKPA, corresponding to amino acid residues 393-405 of rat GABRA2

Product Page - Scientific background :

GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake and metabolism occur in the nervous system.1 The GABA transmitter interacts with two major types of receptors: ionotropic GABAA (GABAAR) and metabotropic GABAB receptors. The GABAAR belong to the ligand gated ion channel superfamily.2 The GABAAR, is a heteropentamer, with all of its five subunits contributing to the pore formation. To date, eight subunit isoforms were cloned: α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunit isoforms were described to exist in mammals (α1-α6). The native GABAA receptor, in most cases, consists of 2α, 2β and 1γ submit. The α subunit is the most common subunit and is expressed ubiquitously. It determines the affinities for allosteric ligands shown by GABAAR. α2-Subunit mRNA is strongly expressed only in the hippocampus and the hypothalamus. It occurs very early in rat brain development and declines in adulthood. The failure to complete the normal transition between the α-subunits that are highly expressed in early development (α2, α3, α5) to those expressed in adulthood (α1) might play a major role in the development of temporal lobe epilepsy.3

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid receptor type A subunit α2, GABRA2

Short Description :

A Rabbit Polyclonal Antibody to GABA(A) α2 Receptor

Long Description :

Alomone Labs offers a highly specific antibody directed against an epitope of the rat GABA (A) α2 receptor. Anti-GABA(A) α2 Receptor Antibody (#AGA-002) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize GABRA2 from human, rat and mouse samples.

Negative Control :

BLP-GA002

Positive Control :

Synonyms :

γ-Aminobutyric acid receptor type A subunit α2, GABRA2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRA2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage