Anti-GABA(A) α1 Receptor (extracellular) Antibody | Alomone Labs AGA-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) α1 Receptor (extracellular) Antibody

catalog :

AGA-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 30

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig 6f	Tamargo Gómez I, Martínez García G, Suarez M, Rey V, Fueyo A, Codina Martínez H, et al. ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration. Cell Death Differ. 2021;: pubmed publisher
western blot; human; 1:1000; loading ...	Pandya M, Palpagama T, Turner C, Waldvogel H, Faull R, Kwakowsky A. Sex- and age-related changes in GABA signaling components in the human cortex. Biol Sex Differ. 2019;10:5 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 8d	Lauritzen K, Hasan Olive M, Regnell C, Kleppa L, Scheibye Knudsen M, Gjedde A, et al. A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain. Neurobiol Aging. 2016;48:34-47 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; fig 2	Gao Y, Heldt S. Enrichment of GABAA Receptor Î±-Subunits on the Axonal Initial Segment Shows Regional Differences. Front Cell Neurosci. 2016;10:39 pubmed publisher
immunohistochemistry; rat; 1:500; loading ...; fig 4c	Wagner F, Bernard R, Derst C, French L, Veh R. Microarray analysis of transcripts with elevated expressions in the rat medial or lateral habenula suggest fast GABAergic excitation in the medial habenula and habenular involvement in the regulation of feeding and energy balance. Brain Struct Funct. 2016;221:4663-4689 pubmed
	Ruiz Reig N, Garc xed a S xe1 nchez D, Schakman O, Gailly P, Tissir F. Inhibitory synapse dysfunction and epileptic susceptibility associated with KIF2A deletion in cortical interneurons. Front Mol Neurosci. 2022;15:1110986 pubmed publisher
	Szocs S, Henn Mike N, Agocs Laboda A, Szabó Meleg E, Varga C. Neurogliaform cells mediate feedback inhibition in the medial entorhinal cortex. Front Neuroanat. 2022;16:779390 pubmed publisher
	Chong J, Cheeseman J, Pawley M, Kwakowsky A, Warman G. The Effects of General Anaesthesia and Light on Behavioural Rhythms and GABAA Receptor Subunit Expression in the Mouse SCN. Clocks Sleep. 2021;3:482-494 pubmed publisher
	Kreis A, Desloovere J, Suelves N, Pierrot N, Yerna X, Issa F, et al. Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission. Sci Rep. 2021;11:17600 pubmed publisher
	Wang R, He R, Jiao H, Zhang R, Guo J, Liu X, et al. Preventive treatment with ginsenoside Rb1 ameliorates monocrotaline-induced pulmonary arterial hypertension in rats and involves store-operated calcium entry inhibition. Pharm Biol. 2020;58:1055-1063 pubmed publisher
	Wiera G, Lebida K, Lech A, Brzd x105 k P, Van Hove I, De Groef L, et al. Long-term plasticity of inhibitory synapses in the hippocampus and spatial learning depends on matrix metalloproteinase 3. Cell Mol Life Sci. 2021;78:2279-2298 pubmed publisher
	Benbow T, Ranjbar Ekbatan M, Cairns B. α1 adrenergic receptor activation has a dynamic effect on masticatory muscle afferent fibers. Neuropharmacology. 2020;175:108197 pubmed publisher
	Sapp E, Seeley C, Iuliano M, Weisman E, Vodicka P, DiFiglia M, et al. Protein changes in synaptosomes of Huntington's disease knock-in mice are dependent on age and brain region. Neurobiol Dis. 2020;141:104950 pubmed publisher
	Nie H, Bai Z, Li Z, Yan L, Chen X. Intravenous lipid emulsion modifies synaptic transmission in hippocampal CA1 pyramidal neurons after bupivacaine-induced central nervous system toxicity. J Neurochem. 2020;154:144-157 pubmed publisher
	Mueller P, Fyk Kolodziej B, Azar T, Llewellyn Smith I. Subregional differences in GABAA receptor subunit expression in the rostral ventrolateral medulla of sedentary versus physically active rats. J Comp Neurol. 2019;: pubmed publisher
	Paul E, Tossell K, Ungless M. Transcriptional profiling aligned with in situ expression image analysis reveals mosaically expressed molecular markers for GABA neuron sub-groups in the ventral tegmental area. Eur J Neurosci. 2019;50:3732-3749 pubmed publisher
	Palpagama T, Sagniez M, Kim S, Waldvogel H, Faull R, Kwakowsky A. GABAA Receptors Are Well Preserved in the Hippocampus of Aged Mice. Eneuro. 2019;6: pubmed publisher
	Vo H, Phillips M, Herskowitz J, King G. Klotho deficiency affects the spine morphology and network synchronization of neurons. Mol Cell Neurosci. 2019;98:1-11 pubmed publisher
	Koga K, Shimoyama S, Yamada A, Furukawa T, Nikaido Y, Furue H, et al. Chronic inflammatory pain induced GABAergic synaptic plasticity in the adult mouse anterior cingulate cortex. Mol Pain. 2018;14:1744806918783478 pubmed publisher
	Sumitomo A, Yukitake H, Hirai K, Horike K, Ueta K, Chung Y, et al. Ulk2 controls cortical excitatory-inhibitory balance via autophagic regulation of p62 and GABAA receptor trafficking in pyramidal neurons. Hum Mol Genet. 2018;27:3165-3176 pubmed publisher
	Orlando M, Ravasenga T, Petrini E, Falqui A, Marotta R, Barberis A. Correlating Fluorescence and High-Resolution Scanning Electron Microscopy (HRSEM) for the study of GABAA receptor clustering induced by inhibitory synaptic plasticity. Sci Rep. 2017;7:13768 pubmed publisher
	Zhang J, Yu J, Kannampalli P, Nie L, Meng H, Medda B, et al. MicroRNA-mediated downregulation of potassium-chloride-cotransporter and vesicular ?-aminobutyric acid transporter expression in spinal cord contributes to neonatal cystitis-induced visceral pain in rats. Pain. 2017;158:2461-2474 pubmed publisher
	de Luca E, Ravasenga T, Petrini E, Polenghi A, Nieus T, Guazzi S, et al. Inter-Synaptic Lateral Diffusion of GABAA Receptors Shapes Inhibitory Synaptic Currents. Neuron. 2017;95:63-69.e5 pubmed publisher
	Doshina A, Gourgue F, Onizuka M, Opsomer R, Wang P, Ando K, et al. Cortical cells reveal APP as a new player in the regulation of GABAergic neurotransmission. Sci Rep. 2017;7:370 pubmed publisher
	Pennacchietti F, Vascon S, Nieus T, Rosillo C, Das S, Tyagarajan S, et al. Nanoscale Molecular Reorganization of the Inhibitory Postsynaptic Density Is a Determinant of GABAergic Synaptic Potentiation. J Neurosci. 2017;37:1747-1756 pubmed publisher
	Burton S, LaRocca G, Liu A, Cheetham C, Urban N. Olfactory Bulb Deep Short-Axon Cells Mediate Widespread Inhibition of Tufted Cell Apical Dendrites. J Neurosci. 2017;37:1117-1138 pubmed publisher
	Redolfi N, Galla L, Maset A, Murru L, Savoia E, Zamparo I, et al. Oligophrenin-1 regulates number, morphology and synaptic properties of adult-born inhibitory interneurons in the olfactory bulb. Hum Mol Genet. 2016;25:5198-5211 pubmed publisher
	Du Z, Chazalon M, Bestaven E, Leste Lasserre T, Baufreton J, Cazalets J, et al. Early GABAergic transmission defects in the external globus pallidus and rest/activity rhythm alteration in a mouse model of Huntington's disease. Neuroscience. 2016;329:363-79 pubmed publisher
	Ghafari M, Falsafi S, Szodorai E, Kim E, Li L, Hoger H, et al. Formation of GABAA receptor complexes containing ?1 and ?5 subunits is paralleling a multiple T-maze learning task in mice. Brain Struct Funct. 2017;222:549-561 pubmed publisher
	Arellano R, SÃ¡nchez GÃ³mez M, Alberdi E, Canedo Antelo M, Chara J, Palomino A, et al. Axon-to-Glia Interaction Regulates GABAA Receptor Expression in Oligodendrocytes. Mol Pharmacol. 2016;89:63-74 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysates: - 1. Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001), (1:1000).2. Anti-GABA (A) α1 Receptor (extracellular) Antibody, preincubated with GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001).

image 2 :

Expression of GABRA1 in mouse hippocampus - Immunohistochemical staining of mouse hippocampus using Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001). A. Distribution of GABRA1 (red). B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that distribution of GABRA1 is restricted to neurons and their processes. DAPI is used as the counterstain (blue).

product information

CAT :

AGA-001

SKU :

AGA-001-CF_0.2 ml

Product Name :

Anti-GABA(A) α1 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P62813

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA001

Homology :

Mouse - identical; human, bovine - 16/17 amino acid residues identical; chicken - 14/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABRA1

Product Page - Scientific background :

GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system.1The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily.2 GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.3 Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.2GABAARs are heteropentamers, in which all five subunits contribute to pore formation. To date, eight subunit isoforms have been cloned:α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consists of 2α, 2β, and 1δ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinities of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes.4 The α1 subunit is highly expressed in adulthood while the α2 subunit is highly expressed very early in rat brain development. Failure to complete the normal transition between the α-subunits that are highly expressed in early development (α2, α3, and α5) and those expressed in adulthood (α1) is suggested to play a major role in the development of temporal lobe epilepsy.5

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid receptor type A subunit α1, GABRA1

Short Description :

A Rabbit Polyclonal Antibody to GABA(A) α1 Receptor

Long Description :

Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry,immunocytochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is highly suited to detect GABRA1 in live cells. It has been designed to recognize GABRA1 from human, rat, and mouse samples.

Negative Control :

BLP-GA001

Positive Control :

Synonyms :

γ-Aminobutyric acid receptor type A subunit α1, GABRA1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC LCI

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage