Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody | Alomone Labs AGA-001-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody

catalog :

AGA-001-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Kreis A, Desloovere J, Suelves N, Pierrot N, Yerna X, Issa F, et al. Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission. Sci Rep. 2021;11:17600 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) membranes and human brain neuroblastoma (SH-SY5Y) (lanes 3 and 6) cell lysate: - 1-3. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP), (1:400).4-6. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody, preincubated with GABA(A) α1 Receptor (extracellular) Blocking Peptide (#BLP-GA001). Following a broad screen of secondary antibodies, the following were used for this application:Western blot analysis:#106-035-006 (Jackson ImmunoResearch) #A7289 (Sigma)

image 2 :

Multiplex staining of KCC2 and GABA(A) α1 Receptor in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using rabbit Anti-KCC2 (SLC12A5) Antibody (#AKT-005), (1:400) and Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP), (1:400). A. KCC2 immunoreactivity (green) is detected in both the granule layer (GL) and the molecular layer (MOL). B. GABA(A) α1 receptor staining (red) is observed mainly in the granule layer (GL). C. Merge of the two images demonstrates colocalization in the granule layer (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AGA-001-GP

SKU :

AGA-001-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P62813

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-GA001

Homology :

Mouse - identical; human - 16/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABRA1

Product Page - Scientific background :

GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system.1The GABA transmitter interacts with two major types of receptors: ionotropic GABAAreceptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily.2 GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.3 Binding of GABA to its GABAA receptor results in conformational changes that open a Cl-channel, producing an increase in membrane conductance that results in inhibition of neural activity.2GABAARs are heteropentamers, in which all five subunits contribute to pore formation. To date, eight subunit isoforms have been cloned:α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consists of 2α, 2β, and 1δ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinities of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes.4 The α1 subunit is highly expressed in adulthood while the α2 subunit is highly expressed very early in rat brain development. Failure to complete the normal transition between the α-subunits that are highly expressed in early development (α2, α3, and α5) and those expressed in adulthood (α1) is suggested to play a major role in the development of temporal lobe epilepsy.5

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid receptor type A subunit α1, GABRA1

Short Description :

A Guinea Pig Polyclonal Antibody to GABA(A) α1 Receptor

Long Description :

Alomone Labs offers a highly specific antibody directed against an extracellular epitope of rat GABA (A) α1 receptor. Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001-GP, formerly AGP-083) raised in guinea pig can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is highly suited to detect GABRA1 in live cells. It has been designed to recognize GABRA1 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-GA001

Positive Control :

Synonyms :

γ-Aminobutyric acid receptor type A subunit α1, GABRA1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage