Anti-GABA(A) α1 Receptor (extracellular)-ATTO Fluor-488 Antibody | Alomone Labs AGA-001-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GABA(A) α1 Receptor (extracellular)-ATTO Fluor-488 Antibody

catalog :

AGA-001-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Wang J, Zhao M, Jia P, Liu F, Chen K, Meng F, et al. The analgesic action of larixyl acetate, a potent TRPC6 inhibitor, in rat neuropathic pain model induced by spared nerve injury. J Neuroinflammation. 2020;17:118 pubmed publisher

image

image 1 :

Multiplex staining of CaV1.2 and GABA(A) α1 Receptor in rat and mouse hippocampus - Immunohistochemical staining of mouse and rat hippocampal dentate gyrus using Guinea pig Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003-GP) and Anti-GABA(A) α1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#AGA-001-AG) in the same section. Both CaV1.2 (red) and GABRA1 (green) are detected in neuron-shaped cells (arrows). Staining suggests partial colocalization between CaV1.2 and GABRA1 in a sub-population of rat dentate gyrus neurons. A. Mouse hippocampus. B. Rat hippocampus.

image 2 :

Immuno-colocalization of CaV1.2 and GABA(A)?1 Receptor in rat cerebellum - Immunohistochemical staining of rat cerebellum using Guinea pigAnti-CaV1.2(CACNA1C)Antibody (#AGP-001) andAnti-GABA(A)?1 Receptor (extracellular)-ATTO-488 Antibody(#AGA-001-AG).A. CaV1.2 (red) is detected mostly in Purkinje cells (arrow). B. In the same sectionGABA(A) ?1 Receptor (green) is observed in the granule layer. C. Merge of the two images suggests some colocalization between CaV1.2 and GABA(A) ?1 Receptor in the rat granule layer but only CaV1.2 appears in Purkinje cells.

image 3 :

Expression of GABRA1 in rat brain - Immunohistochemical staining of rat dentate gyrus usingAnti-GABA(A) ?1 Receptor (extracellular)-ATTO-488Antibody(#AGA-001-AG) (green). Staining reveals hilar neurons (arrows) and fibers coursing through the granule layer (G).DAPI is used as the counterstain(blue).

product information

CAT :

AGA-001-AG

SKU :

AGA-001-AG_50 mcl

Product Name :

Anti-GABA(A) α1 Receptor (extracellular)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P62813

Applications :

IC IF IHC LCI

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GA001

Homology :

Mouse - identical; human, bovine - 16/17 amino acid residues identical; chicken - 14/17 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

QPSQDELKDNTTVFTR(C), corresponding to amino acid residues 28-43 of rat GABRA1

Product Page - Scientific background :

GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system.1The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily.2 GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.3 Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.2GABAARs are heteropentamers, in which all five subunits contribute to pore formation. To date, eight subunit isoforms have been cloned:α, β, γ, δ, ε, π, θ, and ρ.1 Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consists of 2α, 2β, and 1δ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinities of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes.4 The α1 subunit is highly expressed in adulthood while the α2 subunit is highly expressed very early in rat brain development. Failure to complete the normal transition between the α-subunits that are highly expressed in early development (α2, α3, and α5) and those expressed in adulthood (α1) is suggested to play a major role in the development of temporal lobe epilepsy.5

Applications may also work in :

IC IF IHC LCI

Supplier :

Alomone Labs

Target :

γ-Aminobutyric acid receptor type A subunit α1, GABRA1

Short Description :

A Rabbit Polyclonal Antibody to GABA(A) α1 Receptor Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGA-001) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize GABRA1 from human, rat, and mouse samples. Anti-GABA(A) α1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#AGA-001-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-GABA(A) α1 Receptor (extracellular)-ATTO Fluor-488 Antibody has been tested in immunohistochemistry and live cell imaging applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

γ-Aminobutyric acid receptor type A subunit α1, GABRA1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

GABRA1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AGA-001), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage