Anti-SLC19A1/RFC1 (extracellular) Antibody | Alomone Labs AFT-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC19A1/RFC1 (extracellular) Antibody

catalog :

AFT-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat small intestine lysate (lanes 3 and 7) and rat liver membranes (lanes 4 and 8): - 1-4. Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001), (1:400). 5-8. Anti-SLC19A1/RFC1 (extracellular) Antibody, preincubated with SLC19A1/RFC1 (extracellular) Blocking Peptide (#BLP-FT001).

image 2 :

Expression of SLC19A1 in rat hippocampus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001), (1:1200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA3 region, showed immunoreactivity (green) in neuronal profiles in the pyramidal layer (arrows). B. Pre-incubation of the antibody with SLC19A1/RFC1 (extracellular) Blocking Peptide (BLP-FT001), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer.

product information

CAT :

AFT-001

SKU :

AFT-001-CF_0.2 ml

Product Name :

Anti-SLC19A1/RFC1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P41438

Applications :

IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-FT001

Homology :

Rat - 13/15 amino acid residues identical; human - 11/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

6th extracellular loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)SDKRGLGLQVRDQFR, corresponding to amino acid residues 412 - 426 of mouse SLC19A1

Product Page - Scientific background :

Solute Carrier Transporter 19A1, (SLC19A1, RFC1) is a high-capacity, bi-directional transporter of 5-methyl-tetrahydrofolate and thiamine monophosphate1,2. SLC19A1 is characterized by 12 transmembrane domains (TMDs) and cytoplasm oriented N- and C-termini3.Folate is a major nutrient that supports important physiological functions such as DNA synthesis, cell division and substrate methylation. Low folate levels caused by suboptimal intake, transport and cellular utilization of folate, is a risk factor for diseases such as spina bifida, cardiovascular diseases and certain types of cancer1.SLC19A1 is the main folate transporter and it plays a critical role in folate uptake and homeostasis in mammalian cells4. High SLC19A1 transcript levels are detected in liver and placenta, with appreciable levels in other tissues, including kidney, lung, bone marrow, intestine, brain, and portions of the central nervous system5.SLC19A1 is also involved in the uptake of Methotrexate (MTX); one of the leading chemotherapeutic agents with the best demonstrated efficacies against childhood acute lymphoblastic leukemia and hence malfunction of this transporter also effects the efficiency of this type of cancer treatment6.

Applications may also work in :

IFC IHC WB

Supplier :

Alomone Labs

Target :

Solute Carrier Family 19 Member 1, Reduced Folate Carrier 1, Reduced Folate Transporter 1, RFT1, Intestinal folate carrier 1, IFC-1, Reduced Folate Transporter, CHMD, FOLT, REFC, RFC, RFT-1

Short Description :

A Rabbit Polyclonal Antibody to SLC19A1 Transporter

Long Description :

Anti-SLC19A1/RFC1 (extracellular) Antibody (#AFT-001) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize SLC19A1 from human, rat, and mouse samples.

Negative Control :

BLP-FT001

Positive Control :

Synonyms :

Solute Carrier Family 19 Member 1, Reduced Folate Carrier 1, Reduced Folate Transporter 1, RFT1, Intestinal folate carrier 1, IFC-1, Reduced Folate Transporter, CHMD, FOLT, REFC, RFC, RFT-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC19A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1200

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage