Anti-FLRT3 (extracellular) Antibody | Alomone Labs AFR-053 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-FLRT3 (extracellular) Antibody

catalog :

AFR-053

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4): - 1-2. Anti-FLRT3 (extracellular) Antibody (#AFR-053), (1:200). 3-4. Anti-FLRT3 (extracellular) Antibody, preincubated with FLRT3 (extracellular) Blocking Peptide (#BLP-FR053).

image 2 :

Expression of FLRT3 in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-FLRT3 (extracellular) Antibody (#AFR-053), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed immunoreactivity (green) in the granule layer (G) and in the hilus (H, arrows). B. Pre-incubation of the antibody with FLRT3 (extracellular) Blocking Peptide (#BLP-FR053), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AFR-053

SKU :

AFR-053-CF_0.2 ml

Product Name :

Anti-FLRT3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

B1H234

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-FR053

Homology :

Mouse - 14 out of 15 amino acids identical, Human - 13 out of 15 amino acids identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-term.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KQPDIKNPKLTKDQR, corresponding to amino acid residues 387 - 401 of rat FLRT3

Product Page - Scientific background :

The leucine-rich repeat (LRR) superfamily is a diverse protein family that consist of leucine-rich domains (LRR domains). The LRR domain is known to regulate protein-protein interaction or cell adhesion. A Large portion of LRR proteins mediate the differentiation and development of nervous tissues1.The fibronectin leucine-rich transmembrane 3 (FLRT3) protein play an important function during excitatory neuron development including neuron migration, axon guidance, or synapse formation2. FLRT3 gene is encoding single pass transmembrane domain with one extracellular LRR domain and a juxtamembrane fibronectin type 3 (FNIII) module. It was found to be expressed in kidney, brain, pancreases, skeletal muscle, lungs, liver placenta and heart3.FLRT3 display a unique multifunctional property acting as chemorepellent by binding to the Netrin receptor Unc5D and regulating radial migration of subventricular progenitors. In addition, it acts as homophilic adhesion molecule regulating the tangential dispersion of migrating neuron progenitors in the developing cortex4. FLRT3 knock out in mice leads to a variety of malformations, like disorganized basement membrane, failure of embryonic turning and ventral body closure, cardiac bifida and asymmetric development of headfolds5.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Fibronectin Leucine Rich Transmembrane Protein 3, Leucine-rich repeat transmembrane protein FLRT3

Short Description :

A Rabbit Polyclonal antibody to FLRT3 (extracellular)

Long Description :

Anti-FLRT3 (extracellular) Antibody (#AFR-053) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize FLRT3 from mouse, rat and human samples.

Negative Control :

BLP-FR053

Positive Control :

Synonyms :

Fibronectin Leucine Rich Transmembrane Protein 3, Leucine-rich repeat transmembrane protein FLRT3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

FLRT3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage