Anti-GPR43/FFAR2 Antibody | Alomone Labs AFR-032 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR43/FFAR2 Antibody

catalog :

AFR-032

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Tarasiuk A, Talar M, Bulak K, Fichna J. Ghee Butter from Bovine Colostrum Reduces Inflammation in the Mouse Model of Acute Pancreatitis with Potential Involvement of Free Fatty Acid Receptors. Nutrients. 2021;13: pubmed publisher
Salaga M, Bartoszek A, Binienda A, Krajewska J, Fabisiak A, Mosinska P, et al. Activation of Free Fatty Acid Receptor 4 Affects Intestinal Inflammation and Improves Colon Permeability in Mice. Nutrients. 2021;13: pubmed publisher

image

image 1 :

Western blot analysis of rat stomach (lanes 1 and 3) and small intestine (lanes 2 and 4) lysates: - 1,2. Anti-GPR43/FFAR2 Antibody (#AFR-032), (1:500).3,4. Anti-GPR43/FFAR2 Antibody, preincubated with GPR43/FFAR2 Blocking Peptide (#BLP-FR032).

image 2 :

Expression of GPR43/FFAR2 in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR43/FFAR2 Antibody (#AFR-032), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA1 region showed FFAR2 immunoreactivity (green) in the pyramidal layer (P) and in astrocyte outlines (arrows). B. Pre-incubation of the antibody with GPR43/FFAR2 Blocking Peptide (BLP-FR032), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

AFR-032

SKU :

AFR-032-CF_0.2 ml

Product Name :

Anti-GPR43/FFAR2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q76EI6

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-FR032

Homology :

Mouse -12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RGAEETVEGTKTDR, corresponding to amino acid residues 301-314 of rat FFAR2

Product Page - Scientific background :

Fatty acids have long been recognized for the variety of their effects in the body. Yet, until recently, these actions were thought to be mediated exclusively via actions on cellular metabolism. There are currently three known receptors for free fatty acids: FFA1, FFA2, and FFA3.Free fatty acid receptor 2 (FFA2) is a G-protein coupled receptor containing seven hydrophobic regions, consistent with transmembrane (TM) spanning helices. FFA2 contains cysteine residues in the first and second extracellular loops that are likely to contribute to structure via the formation of intramolecular disulfide bonds. FFA2 mRNA is detected in a number of tissues, but the highest expression is found in immune cells such as neutrophils, monocytes, peripheral blood mononuclear cells, B-lymphocytes, and polymorphonuclear cells.Short chain fatty acids (SCFAs) are fatty acids of less than six carbons and were found to be endogenous agonists of FFA2. The most potent agonist of the receptor is acetate (C2) and propionate (C3) being equipotent followed by butyrate (C4) and then valerate (C5) and formate (C1)1.FFA2 is implicated in the pathogenesis of several diseases. Stimulation of FFA2 by acetate allows resolution of a colitis-related inflammatory response. FFA2 knockout mice show exacerbated or unresolved inflammation in cases of acute and chronic colitis, arthritis, and asthma. In contrast, other studies show that knockout mice exhibit an increased mortality compared to control mice, despite reduced immune cell recruitment, decreased colonic inflammation, and attenuated colonic tissue damage2.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

G-protein coupled receptor 43, Free fatty acid receptor 2

Short Description :

A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 2 (GPR43)

Long Description :

Anti-GPR43/FFAR2 Antibody (#AFR-032) is a highly selective antibody directed against an epitope of the rat Free fatty acid receptor 2. The antibody can be used in western blot analysis. It has been designed to recognize FFAR2 from mouse and rat samples. The antibody is not suited to recognize FFAR2 from human samples.

Negative Control :

BLP-FR032

Positive Control :

Synonyms :

G-protein coupled receptor 43, Free fatty acid receptor 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

FFAR2

AB Specifictiy :

Will not recognize human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage