Anti-Frizzled-3 (FZD3) Antibody | Alomone Labs AFR-023 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Frizzled-3 (FZD3) Antibody

catalog :

AFR-023

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

product information

CAT :

AFR-023

SKU :

AFR-023-CF_0.2 ml

Product Name :

Anti-Frizzled-3 (FZD3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q61086

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-FR023

Homology :

Human, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KVSSYHGSLHRSRDGR, corresponding to amino acid residues 581 - 596 of mouse Frizzled-3

Product Page - Scientific background :

Frizzled 3, encoded by FZD3 gene, is a receptor in the Wnt/β-catenin pathway, a conserved signaling pathway that regulates gene expression and controls diverse developmental processes 1. It belongs to the Frizzled protein family that contains multiple hydrophobic domains predicted to form seven transmembrane helices, similar to G protein coupled receptors. Conserved sequences are found in the Frizzled proteins extracellular cysteine-rich domain (CRD), the putative transmembrane domains, parts of the intracellular loops and a juxtamembrane motif, KTXXXW, found in the membrane-proximal C-terminal region of all Frizzleds. The C-terminal tails, however, are highly divergent 2. Frizzled-3 is constitutively expressed in murine melanocyte precursors, melanocytes and human melanocytes 3, it is also expressed in the epidermis of the developing embryo 4 and its expression levels are changing between different parts of the ovary throughout the process of embryogenesis 1. Because of its crucial role in regulating proper embryogenesis and its role in the development of the central nervous system, including synaptogenesis and structural plasticity, mutations in FZD3 gene were shown to have a significant association with the development of schizophrenia and other psychotic disorders 5.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Frizzled Class Receptor 3, Fz-3

Short Description :

A Rabbit Polyclonal antibody to Frizzled-3 receptor.

Long Description :

Anti-Frizzled-3 (FZD3) Antibody (#AFR-023) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Frizzled-3 from mouse, rat and human samples.

Negative Control :

BLP-FR023

Positive Control :

Synonyms :

Frizzled Class Receptor 3, Fz-3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

FZD3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage