Anti-Frizzled-2 (FZD2) (extracellular) Antibody | Alomone Labs AFR-022 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Frizzled-2 (FZD2) (extracellular) Antibody

catalog :

AFR-022

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat heart lysate: - 1. Anti-Frizzled-2 (FZD2) (extracellular) Antibody (#AFR-022), (1:200).2. Anti-Frizzled-2 (FZD2) (extracellular) Antibody, preincubated with Frizzled-2/FZD2 (extracellular) Blocking Peptide (#BLP-FR022).

image 2 :

Western blot analysis of human MDA-231 breast carcinoma cell line lysate (lanes 1 and 3) and human THP-1 acute monocytic leukemia cell line lysate (lanes 2 and 4): - 12.Anti-Frizzled-2 (FZD2) (extracellular) Antibody(#AFR-022) (1:200).34. Anti-Frizzled-2 (FZD2) (extracellular) Antibody preincubated with the negative control antigen.

image 3 :

Western blot analysis of rat heart lysate: - 1.Anti-Frizzled-2 (FZD2) (extracellular) Antibody(#AFR-022) (1:200).2. Anti-Frizzled-2 (FZD2) (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

AFR-022

SKU :

AFR-022-CF_0.2 ml

Product Name :

Anti-Frizzled-2 (FZD2) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q08464

Applications :

IFC LCI WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-FR022

Homology :

Human - identical; mouse - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GAPPRYATLEHPFH, corresponding to amino acid residues 192-205 of rat Frizzled-2

Product Page - Scientific background :

Frizzled receptors (FZD) belong to the superfamily of G-protein coupled receptors (GPCR). They are classified to the frizzled subfamily for which 11 members have been identified to date; FZD1-10 and Smoothened1. They were first identified in Drosophila melanogaster.Like all GPCRs, Frizzled receptors have seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. However, these receptors lack many features of the conventional class A, B or C GPCRs2.Activation of these receptors by WNT, leads to numerous complex signaling pathways which are generally termed β-catenin-dependent and β-catenin-independent. It is noteworthy to mention that the β-catenin-dependent pathway involves the interaction of FZD with LRP6 or LRP5 co-receptors3.Frizzled receptors are ubiquitously expressed and play a crucial role in embryo development. They are also significantly involved in proliferation and differentiation. Indeed, dysregulation of FZD leads to various forms of cancer1. Scientific evidence also shows their involvement in axonal remodeling and neurotransmitter release1.They have been associated to Alzheimer's and Parkinson's diseases, depression, anxiety, hypoxia and ischemia. It is thus not surprising that they have become pharmacological targets in a number of diseases and disorders.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

Frizzled class receptor 2, Fz-2

Short Description :

A Rabbit Polyclonal Antibody to Frizzled-2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Frizzled-2. Anti-Frizzled-2 (FZD2) (extracellular) Antibody (#AFR-022) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize FZD2 from rat, mouse and human samples.

Negative Control :

BLP-FR022

Positive Control :

Synonyms :

Frizzled class receptor 2, Fz-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

FZD2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage