Anti-Human GPR43/FFAR2 (extracellular) Antibody | Alomone Labs AFR-015 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human GPR43/FFAR2 (extracellular) Antibody

catalog :

AFR-015

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human HT-29 colon adenocarcinoma cell line (lanes 1 and 5), human HL-60 promyelocytic leukemia cell line (lanes 2 and 6), human THP-1 monocytic leukemia cell line (lane 3 and 7) and human LoVo colorectal adenocarcinoma cell line (lanes 4 and 8) lysates: - 1-4. Anti-Human GPR43/FFAR2 (extracellular) Antibody (#AFR-015), (1:200). 5-8. Anti-Human GPR43/FFAR2 (extracellular) Antibody, preincubated with Human GPR43/FFAR2 (extracellular) Blocking Peptide (#BLP-FR015).

product information

CAT :

AFR-015

SKU :

AFR-015-CF_0.2 ml

Product Name :

Anti-Human GPR43/FFAR2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O15552

Applications :

IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-FR015

Homology :

Human only

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)TEQVRSGNEIT(S)YEN, corresponding to amino acid residues 153-167 of human FFAR2

Product Page - Scientific background :

Free fatty acids (FFAs) are essential nutrients that are also involved in signal transduction through FFA receptors, members of the G-protein coupled receptor superfamily.FFAR2/GPR43 is an FFA receptor that is activated specifically by short fatty acids (acetate, propionate and butyrate), that are the products of bacterial fermentation in the gut1.GPR43 is expressed in adipose tissue, intestines, and immune tissues2 and its stimulation by interaction with short fatty acids inhibits cAMP production, activates the extracellular signal-regulated kinase (ERK) cascade via interactions with the Gi/o family of G-proteins. This increases intracellular Ca2+ levels, and promotes activation of the mitogen-activated protein kinase (MAPK) cascade through interactions with the Gq family of G-proteins1.These processes, mediated by GPR43 receptor regulate the host energy homeostasis and hence GPR43 is shown to be related to metabolic disorders, such as obesity and diabetes1.Because of its pivotal role in energy homeostasis and in regulating many cellular processes, GPR43 has many phenotypic effects: mutant mice are hyper susceptible to developing intestinal carcinogenesis3, Klebsiella pneumoniae infection4 and to increased differentiation of bone marrow cells into osteoclasts5. Studies have shown the role of GPR43 in regulating inflammatory responses, which can lead to colitis and asthma6,7. The various activities mediated by GPR43 makes it an appealing therapeutic target.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

G-protein coupled receptor 43, Free fatty acid receptor 2

Short Description :

A Rabbit Polyclonal Antibody to Human Free Fatty Acid Receptor 2 (GPR43)

Long Description :

Anti-Human GPR43/FFAR2 (extracellular) Antibody (#AFR-015) is a highly specific antibody directed against an epitope of the human Free fatty acid receptor 2. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize FFAR2 from human samples only. It will not recognize the protein from rat or mouse samples.

Negative Control :

BLP-FR015

Positive Control :

Synonyms :

G-protein coupled receptor 43, Free fatty acid receptor 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

FFAR2

AB Specifictiy :

Not recommended for mouse or rat samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage