Anti-GPR120/FFAR4 (extracellular) Antibody | Alomone Labs AFR-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR120/FFAR4 (extracellular) Antibody

catalog :

AFR-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Expression of FFAR4 in rat hypothalamus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (1:1200), followed by biotinylated donkey anti-rabbit and streptavidin-Cy3. A. FFAR4 immunoreactivity (red) appears in axons beaded with varicosities (arrows). B. Pre-incubation of the antibody with GPR120/FFAR4 (extracellular) Blocking Peptide (BLP-FR014), suppressed staining. Cell nuclei are stained with DAPI (blue). VMH = ventromedial hypothalamus, 3V = 3rd ventricle

product information

CAT :

AFR-014

SKU :

AFR-014-CF_0.2 ml

Product Name :

Anti-GPR120/FFAR4 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q7TMA4

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-FR014

Homology :

Rat - identical; human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RTHFPFFSDVKGDHR, corresponding to amino acid residues 22-36 of mouse FFAR4

Product Page - Scientific background :

Free fatty acid receptor 4 (FFAR4, GPR120), is a member of the rhodopsin family of 7-transmembrane domain G-protein coupled receptors (GPCRs). GPR120/FFAR4 is activated by long chain fatty acids. The protein is a potential therapeutic target for the treatment of type 2 diabetes mellitus (T2DM). GPR120 is associated to insulin sensitizing, anti-inflammatory, and fat metabolism. Evidence shows that GPR120 may be involved in the development of obesity in mice and humans1-4. GPR120 regulates various physiological processes, including gut hormone secretion, islet function, osteoclastogenesis, anti-inflammation, and adipogenesis2-4.GPR120 binds to omega-3 fatty acids and stabilizes the metabolic homeostasis through a cascade of physiological activities. GPR120 exerts its physiological effects through one of two pathways that involves either Gαq or β-arrestin2,3.GPR120 has been shown to be ubiquitously expressed and is primarily detected in the intestine, adipocytes, and pro-inflammatory macrophages1,2.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1

Short Description :

A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 4 (GPR120)

Long Description :

Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014) is a highly specific antibody directed against an epitope of the mouse Free fatty acid receptor 4. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize FFAR4 from rat, mouse, and human samples.

Negative Control :

BLP-FR014

Positive Control :

Synonyms :

G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

FFAR4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage