Anti-GPR40/FFAR1 (extracellular) Antibody | Alomone Labs AFR-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GPR40/FFAR1 (extracellular) Antibody

catalog :

AFR-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat pancreas lysate: - 1. Anti-GPR40/FFAR1 (extracellular) Antibody (#AFR-011), (1:200).2. Anti-GPR40/FFAR1 (extracellular) Antibody, preincubated with GPR40/FFAR1 (extracellular) Blocking Peptide (#BLP-FR011).

image 2 :

Expression of GPR40/FFAR1 in rat brain - Immunohistochemical staining of rat brain sections using Anti-GPR40/FFAR1 (extracellular) Antibody (#AFR-011). A. GPR40 immunoreactivity (green) in the cerebellum appears in the molecular layer (MOL) and in Purkinje cells (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).

image 3 :

Cell surface detection of SERT in live intact human MEG-01 megakaryoblastic leukemia cells: - ___Cells.___ Cells + rabbit IgG isotype control-FITC.___Cells + Anti-Serotonin Transporter (SERT) (extracellular)-FITC Antibody (#AMT-004-F) (5 g).

product information

CAT :

AFR-011

SKU :

AFR-011-CF_0.2 ml

Product Name :

Anti-GPR40/FFAR1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8K3T4

Applications :

IF IFC IHC LCI WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-FR011

Homology :

Mouse - 15/16 amino acid residues identical; human - 12/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)NVASFINPDLEGSWRK corresponding to amino acid residues 244-259 of rat FFAR1

Product Page - Scientific background :

Free fatty acids (FFAs) were for long believed to influence cellular metabolism. Hoever, the discovery that FFAs actually mediate their effects through one of three G-protein coupled receptors (GPCRs) - Free Fatty Acid Receptors 1-3 (FFAR1-3), has initiated a number of studies in order to assess their implication in health and disease1.Like all other GPCRs, FFAR1-3 have seven transmembrane domains, an extracellular N-terminal tail and an intracellular C-terminus.FFAR1 is activated by either medium or long, saturated or unsaturated fatty acids2,3, whereas FFAR2 and FFAR3 are activated by short chain fatty acids4,5. FFAR1 is coupled to Gq and leads to Ca2+ mobilization upon activation. FFAR2 couples to both pertussis-sensitive and insensitive G-proteins and also leads to increased intracellular Ca2+ levels. FFAR3 coupling to G-protein induces an increase in cAMP1.Distribution studies of all three FFA Receptors indicate that FFAR1 mRNA is quite elevated in the pancreas2,3. FFAR1 is also detected in the liver, heart, skeletal muscle, and brain; although this broad localization pattern is supported only by one study2. FFAR2 is expressed in many tissues like liver and colon6. FFAR3 is expressed in many tissues but shows highest expression in adipose tissue4. Relatively high expression of the receptor could be detected in pancreas, spleen, lymph nodes and bone marrow4,5.As mentioned above, the finding that FFAs' actions are mediated by GPCRs, prompted many studies in order to elucidate their roles in healthy and disease states. Such studies stipulate that FFAR1 may be involved in obesity and type 2 diabetes since circulating free fatty acid levels in the plasma are significantly elevated. On the other hand, FFAR2 and FFAR3 could be potential targets for treating irritable bowel disease and appetite control1.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

G-protein coupled receptor 40, Free fatty acid receptor 1

Short Description :

A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 1 (GPR40)

Long Description :

Anti-GPR40/FFAR1 (extracellular) Antibody (#AFR-011) is a highly specific antibody directed against an epitope of the rat Free fatty acid receptor 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize FFAR1 from mouse, rat, and human samples.

Negative Control :

BLP-FR011

Positive Control :

Synonyms :

G-protein coupled receptor 40, Free fatty acid receptor 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

FFAR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage