Anti-FSHR (extracellular) Antibody | Alomone Labs AFR-010 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-FSHR (extracellular) Antibody

catalog :

AFR-010

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat ovary (lane 1 and 4), rat testis (lane 2 and 5) and human OVCAR3 (lane 3 and 6) lysates: - 1-3. Anti-FSHR (extracellular) Antibody (extracellular) Antibody (#AFR-010), (1:200).4-6. Anti-FSHR (extracellular) Antibody, preincubated with FSHR (extracellular) Blocking Peptide (#BLP-FR010).

image 2 :

Peptide (C)SNTGIKHLPAVHK corresponding to amino acid residues 128-140 of rat FSHR (AccessionP20395). Extracellular N-terminus.

image 3 :

Expression of FSH receptorin human ovary cell line - Cell surface detection ofFSH receptor in intact living human OVCAR3 cells. A. Extracellular staining of cells using Anti-FSHR (extracellular) Antibody(#AFR-010) (1:25) followed by goat anti-rabbit AlexaFluor-594 secondary antibody. B. Nuclear staining of cells using DAPI as the counterstain. C. Merged images of A and B.

product information

CAT :

AFR-010

SKU :

AFR-010-CF_0.2 ml

Product Name :

Anti-FSHR (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P20395

Applications :

IC IF IHC LCI WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-FR010

Homology :

Mouse, human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SNTGIKHLPAVHK, corresponding to amino acid residues 128-140 of rat FSHR

Product Page - Scientific background :

FSH (follicle stimulating hormone) is the central hormone of mammalian reproduction, necessary for gonadal development and maturation at puberty and for gamete production during the fertile phase of life1. Together with LH, this gonadotropin is produced and secreted by the pituitary gland as a highly heterogeneous glycoprotein2. The FSH receptor belongs to the family of G-protein coupled receptors, complex transmembrane proteins characterized by seven hydrophobic helices inserted in the plasma membrane and by intracellular and extracellular domains of variable dimensions depending on the type of ligand3. The intracellular portion of the FSH receptor is coupled to a Gs protein and, upon receptor activation by the hormonal interaction with the extracellular domain it initiates a cascade of events that ultimately leads to the specific biological effects of the gonadotropin.FSH acts by binding to specific receptors, localized exclusively in the gonads. The FSH receptor is expressed by two cell types of the gonad, namely Sertoli cells in the testis, and granulosa cells in the ovarian follicle4.Mutations of FSHR may cause primary or secondary amenorrhea, infertility, premature ovarian failure (POF) and ovarian hyperstimulation syndrome (OHSS)5. Mutations of FSHR may also play a role in the development of granulosa cell tumors of ovaries6.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

FSH receptor, Follicle-stimulating hormone receptor, Follitropin receptor, FSHRO

Short Description :

A Rabbit Polyclonal Antibody to FSH Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat FSH receptor. Anti-FSHR (extracellular) Antibody (#AFR-010) can be used in western blot and live cell imaging applications. It has been designed to recognize the FSH receptor from rat, mouse and human samples.

Negative Control :

BLP-FR010

Positive Control :

Synonyms :

FSH receptor, Follicle-stimulating hormone receptor, Follitropin receptor, FSHRO

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

FSHR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage