Anti-Human FPR1 (extracellular) Antibody | Alomone Labs AFR-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human FPR1 (extracellular) Antibody

catalog :

AFR-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Tarasiuk A, Talar M, Bulak K, Fichna J. Ghee Butter from Bovine Colostrum Reduces Inflammation in the Mouse Model of Acute Pancreatitis with Potential Involvement of Free Fatty Acid Receptors. Nutrients. 2021;13: pubmed publisher
Salaga M, Bartoszek A, Binienda A, Krajewska J, Fabisiak A, Mosinska P, et al. Activation of Free Fatty Acid Receptor 4 Affects Intestinal Inflammation and Improves Colon Permeability in Mice. Nutrients. 2021;13: pubmed publisher

image

image 1 :

Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 5), human THP-1 acute monocytic leukemia (lanes 2 and 6), human T-84 colorectal carcinoma (lanes 3 and 7) and human U-87 MG glioblastoma (lanes 4 and 8) cell lysates: - 1-4. Anti-Human FPR1 (extracellular) Antibody (#AFR-001), (1:200).5-8. Anti-Human FPR1 (extracellular) Antibody, preincubated with Human FPR1 (extracellular) Blocking Peptide (#BLP-FR001).

image 2 :

Peptide (C)NFSPWTNDPKERIN corresponding to amino acid residues 179-192 of human FPR1 (AccessionP21462). 2ndextracellular loop.

image 3 :

Cell surface detection of FPR1 in live intact human THP-1 acute monocytic leukemia cell line: - ___Unstained cells + goat-anti-rabbit-Cy5.___Cells +Anti-Human FPR1 (extracellular) Antibody (#AFR-001) (1:25) + goat-anti-rabbit-Cy5.

product information

CAT :

AFR-001

SKU :

AFR-001-CF_0.2 ml

Product Name :

Anti-Human FPR1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P21462

Applications :

IFC LCI WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-FR001

Homology :

Human only Not recommended for use with rat and mouse samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)NFSPWTNDPKERIN, corresponding to amino acid residues 179-192 of human FPR1

Product Page - Scientific background :

Chemotactic factors from both Gram-positive and Gram-negative bacteria are short peptides with N-formyl methionine at the N-terminus (extensively reviewed in reference 1). These peptides are released from bacteria during infection and activate formyl peptide receptors (FPR), members of the G-protein coupled receptor (GPCR) superfamily. In humans, the FPR family consists mainly of three receptors, FPR1, FPR2/ALX (formerly FPRL1), and FPR3 (formerly FPRL2) which all couple to the Gi subtype of G-proteins and ultimately lead to the activation of phospholipase C and intracellular Ca2+ increase1,2.FPRL1, or FPR2/ALX as it is commonly called, is a seven transmembrane protein like all GPCRs. This receptor was originally cloned by screening a HL60 neutrophil cDNA library with a FPR1 cDNA probe3. FPR2/ALX shares 69% identity with FPR1 and despite its high homology, it displays relatively low affinity for fmlf, the most potent N-formyl peptide released by bacteria3.FPR1 was originally found in neutrophils and later found to be distributed in myeloid and non-myeloid cells as is the case for FPR2/ALX and FPR3 (FPR3 though is not expressed in neutrophils). FPR1 is also expressed in multiple organs and tissues including epithelial cells in organs with secretory functions, endocrine cells, liver hepathocytes, smooth muscle cells and endothelial cells, brain spinal cord and both motor and sensory neurons4. FPR2/ALX has a similar tissue distribution to that of FPR1.While N-formyl peptides were the first peptides found to activate these receptors, the ligand diversity for FPR has proven to be quite broad and demonstrates to be both pro- and anti-inflammatory. They include peptidic ligands originating from bacterial and viral sources (including HIV), endogenous ligands such as chemokines and annexins, short peptides associated with inflammation and infection. Indeed, peptides from Herpes, Ebola and coronavirus 229E are ligands of FPR11.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

N-formyl peptide receptor 1, fMLP receptor

Short Description :

A Rabbit Polyclonal Antibody to Human FPR1

Long Description :

Anti-Human FPR1 (extracellular) Antibody (#AFR-001) is a highly specific antibody directed against an epitope of the human N-formyl peptide receptor 1. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize FPR1 from human samples only.

Negative Control :

BLP-FR001

Positive Control :

Synonyms :

N-formyl peptide receptor 1, fMLP receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

FPR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage